Optimization of a nanotechnology based antimicrobial platform for food safety applications using Engineered Water Nanostructures (EWNS)

Details

• Personal Author:
  Cirenza C ; Demokritou P ; Eleftheriadou M ; Leonard, Stephen S. ; McDevitt J ; Pyrgiotakis G ; Vedantam P
• Description:
  A chemical free, nanotechnology-based, antimicrobial platform using Engineered Water Nanostructures (EWNS) was recently developed. EWNS have high surface charge, are loaded with reactive oxygen species (ROS), and can interact-with, and inactivate an array of microorganisms, including foodborne pathogens. Here, it was demonstrated that their properties during synthesis can be fine tuned and optimized to further enhance their antimicrobial potential. A lab based EWNS platform was developed to enable fine-tuning of EWNS properties by modifying synthesis parameters. Characterization of EWNS properties (charge, size and ROS content) was performed using state-of-the art analytical methods. Further their microbial inactivation potential was evaluated with food related microorganisms such as Escherichia coli, Salmonella enterica, Listeria innocua, Mycobacterium parafortuitum, and Saccharomyces cerevisiae inoculated onto the surface of organic grape tomatoes. The results presented here indicate that EWNS properties can be fine-tuned during synthesis resulting in a multifold increase of the inactivation efficacy. More specifically, the surface charge quadrupled and the ROS content increased. Microbial removal rates were microorganism dependent and ranged between 1.0 to 3.8 logs after 45 mins of exposure to an EWNS aerosol dose of 40,000 #/cm3. [Description provided by NIOSH]
• Subjects:
  Aerosols Laboratories Microbiology Occupational Health Safety
• Keywords:
  Analytical Methods Analytical Processes Antimicrobials Food Food Contaminants Laboratory Techniques Laboratory Testing Microorganisms Mycobacterium Nanostructures Nanotechnology Pathogens Reactive Oxygen Species ROS Surface Properties

  More +
• ISSN:
  2045-2322
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Massachusetts ; OSHA Region 1 ; OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  6
• NIOSHTIC Number:
  nn:20047954
• Citation:
  Sci Rep 2016 Feb; 6:21073
• Contact Point Address:
  Philip Demokritou, Center for Nanotechnology and Nanotoxicology, Harvard School of Public Health, Boston, MA, USA
• Email:
  pdemokri@hsph.harvard.edu
• Federal Fiscal Year:
  2016
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  True
• Source Full Name:
  Scientific Reports
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:1a22174117c2a4023223c2b44778955f09af8dbd5d8ab690f3fdd080e3796a1c8d96282089f93bc8fb475b98c7136450987861519f887e026ab7680a2147ddd8
• Download URL:
  https://stacks.cdc.gov/view/cdc/202874/cdc_202874_DS1.pdf
• File Type:
  [PDF - 1.56 MB ]