Nano-scaled cerium oxide induces platelet activation in vivo

Details

• Personal Author:
  Barger M ; Cohen J ; Davidson DC ; Demokritou P ; Derk R ; Ma J ; Stueckle, Todd A. ; Wang L
• Description:
  Nano-scaled cerium oxide (nCeO2) is used in a variety of applications, including use as a fuel additive, catalyst, and polishing agent, yet potential adverse health effects associated with nCeO2 exposure remain incompletely understood. In vivo studies using a rat model have shown that inhaled nCeO2 can deposit in deep lung tissues and induce fibrosis; however, little is known about other potential cardiovascular disorders (CVD) associated with nCeO2 exposure. Similarly, blood-based biomarkers to predict risk of nCeO2-induced disease remain limited. To address these knowledge gaps, rats were intratracheally instilled with 3.5 mg/kg nCeO2 and plasma samples were analyzed 28 days after treatment to identify potential biomarkers of such nCeO2-induced disorders. Plasma samples revealed increased levels of pro-inflammatory mediators such as IL-1beta and IL-6, as well as the pro-fibrogenic mediator TGFbeta1 in response to nCeO2. Platelets are one of the main sources of circulating TGFbeta1, and these small (3-5 microm), anucleate cells are known to contribute to numerous inflammatory disorders and CVD following activation and subsequent degranulation. Thus, we hypothesized that platelets are activated in response to nCeO2 treatment. Consistently, we found that platelets isolated from nCeO2-treated rats released significantly more TGFbeta1 than those isolated from control animals, indicating degranulation of these cells and suggesting that platelets may be contributing to nCeO2-induced fibrosis and CVD. Interestingly, an amorphous silica-coated nCeO2 (amsCeO2) induced levels of TGFbeta1 from platelets consistent with those isolated from saline-treated animals, demonstrating that "safety-by-design" strategies for preparing engineered nanomaterials may prove useful in ameliorating some of the harmful effects of nCeO2. Collectively, these results shed light on possible biomarkers for nCeO2-incuded disease and highlight a potential role for platelets in such diseases. Disclaimer: The findings and conclusions in this abstract are those of the authors and do not necessarily represent the views of the National Institute for Occupational Safety and Health. [Description provided by NIOSH]
• Subjects:
  Biomarkers Blood Cardiovascular Diseases Cardiovascular System Fibrosis Hematologic Tests Immune System Laboratories Occupational Health Safety Toxicology

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• Keywords:
  Amorphous Silica Blood Plasma Blood Samples Cardiovascular Disease Cardiovascular System Disorders Cellular-reactions Cerium-compounds Coatings Dose-response Fibrogenicity Immune Reaction Industrial Design Industrial Engineering Laboratory-animals Laboratory-testing Nanomaterials Nanoscale Nanotechnology Platelets

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  California ; Massachusetts ; OSHA Region 1 ; OSHA Region 3 ; OSHA Region 9 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  115-116
• Volume:
  150
• Issue:
  1
• NIOSHTIC Number:
  nn:20047615
• Citation:
  Toxicologist 2016 Mar; 150(1):115-116
• CAS Registry Number:
  Ceria (CAS RN 1306-38-3) ; Silica (CAS RN 7631-86-9)
• Federal Fiscal Year:
  2016
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 55th Annual Meeting and ToxExpo, March 13-17, 2016, New Orleans, Louisiana
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:59eacf3cf6b55990804eba4fbf42d9d949b9228f8ff25a80a447956ebdec8ba210eb2efe3b4c164c6b0e25de2d422226c4e88bffb0aaebb247fef4f1e90e1afa
• Download URL:
  https://stacks.cdc.gov/view/cdc/202630/cdc_202630_DS1.pdf
• File Type:
  [PDF - 452.00 KB ]