Application of ATP-based bioluminescence for bioaerosol quantification: effect of sampling method

Details

• Personal Author:
  DuBois K ; Han T ; Mainelis G ; Therkorn J ; Wren M
• Description:
  An adenosine triphosphate (ATP)-based bioluminescence has potential to offer a quick and affordable method for quantifying bioaerosol samples. Here we report on our investigation into how different bioaerosol aerosolization parameters and sampling methods affect bioluminescence output per bacterium, and implications of that effect for bioaerosol research. Bacillus atrophaeus and Pseudomonas fluorescens bacteria were aerosolized by using a Collison nebulizer (BGI Inc., Waltham, MA) with a glass or polycarbonate jar and then collected for 15 and 60 min with: (1) Button Aerosol Sampler (SKC Inc., Eighty Four, PA) with polycarbonate, PTFE, and cellulose nitrate filters, (2) BioSampler (SKC Inc.) with 5 and 20 mL of collection liquid, and (3) our newly developed Electrostatic Precipitator with Superhydrophobic Surface (EPSS). For all aerosolization and sampling parameters we compared the ATP bioluminescence output per bacterium relative to that before aerosolization and sampling. In addition, we also determined the ATP reagent storage and preparation conditions that that do not affect the bioluminescence signal intensity. Our results show that aerosolization by a Collison nebulizer with a polycarbonate jar yields higher bioluminescence output per bacterium compared to the glass jar. Interestingly enough, the bioluminescence output by P. fluorescens increased substantially after its aerosolization compared to the fresh liquid suspension. For both test microorganisms, the bioluminescence intensity per bacterium after sampling was significantly lower than that before sampling suggesting negative effect of sampling stress on bioluminescence output. The decrease in bioluminescence intensity was more pronounces for longer sampling times and significantly and substantially depended on the sampling method. Among the investigated method, the EPSS was the least injurious for both microorganisms and sampling times. While the ATP-based bioluminescence offers a quick bioaerosol sample analysis method, this works demonstrates that the method output depends on bioaerosol generation and sampling methods, as well as reagent storage. [Description provided by NIOSH]
• Subjects:
  Aerosols Microbiology Occupational Health Safety
• Keywords:
  Aerosolization Author Keywords: ATP Bioluminescence Bacteria Bioaerosols Biological Function Microorganisms Samplers Sampling Sampling Methods Sampling Stress Stress

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• ISSN:
  0021-8502
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  New Jersey ; OSHA Region 2
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  114-123
• Volume:
  90
• NIOSHTIC Number:
  nn:20047375
• Citation:
  J Aerosol Sci 2015 Dec; 90:114-123
• Contact Point Address:
  Gediminas Mainelis, Department of Environmental Sciences, Rutgers, The State University of New Jersey, 14 College Farm Road, New Brunswick, NJ 08901
• Email:
  mainelis@envsci.rutgers.edu
• Federal Fiscal Year:
  2016
• Performing Organization:
  Rutgers, The State University of New Jersey, Piscataway
• Peer Reviewed:
  True
• Start Date:
  20100801
• Source Full Name:
  Journal of Aerosol Science
• End Date:
  20190731
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:09a6bf82809e7a286c3c8e094a8a877265b61c4ba2a487d8b51dd48df2864bd6e953e732070b9a597b542514fe9b4058e9812733208b66141833597bb8aac888
• Download URL:
  https://stacks.cdc.gov/view/cdc/202459/cdc_202459_DS1.pdf
• File Type:
  [PDF - 901.78 KB ]