Improved Methods for Generation, Sampling, and Recovery of Biological Aerosols in Filter Challenge Tests

Details

• Personal Author:
  Brosseau LM ; McCullough NV ; Vesley D ; Vincent JH
• Description:
  In preparation for filter efficiency tests and sampler comparison studies, methods of biological aerosol generation, sampling, and filter recovery were modified from previous studies. Methods described include (1) techniques for generating aerosols that reduced nuisance particles to negligible levels and increased the cell culturability of Mycobacterium abscessus by 30%, (2) sampling techniques that lowered the detectable range of biological particle size from 0.65 to 0.45 micron and reduced the sampling flow from the chamber from 28.3 to 1.5 L/min, and (3) development of methods to remove culturable organisms from respirator filter media. These methods were developed for filter challenge tests with M. abscessus and were applied to two other bacteria. They may also have application to a wider variety of organisms and bioaerosol assessments. [Description provided by NIOSH]
• Subjects:
  Aerosols Bacteria Dust Microbiology Occupational Health Particulate Matter Safety
• Keywords:
  Aerosol-sampling Author Keywords: Aerosols Bacterial-dusts Filter Efficiency Filters Microorganisms Mycobacterium Abscessus Particle Size Particulate-sampling-methods Samplers
• ISSN:
  0002-8894
• Document Type:
  Text
• Funding:
  Contract
• Genre:
  Journal Article
• Place as Subject:
  Minnesota ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  234-241
• Volume:
  59
• Issue:
  4
• NIOSHTIC Number:
  nn:20023159
• Citation:
  Am Ind Hyg Assoc J 1998 Apr; 59(4):234-241
• Contact Point Address:
  University of Minnesota, School of Public Health, Division of Environmental and Occupational Health, Box 807 Mayo, 420 Delaware St. NE, Minneapolis, MN 55455
• Federal Fiscal Year:
  1998
• NORA Priority Area:
  Research Tools and Approaches: Exposure Assessment Methods
• Performing Organization:
  Division of Environmental and Occupational Health, School of Public Health, University of Minnesota, Minneapolis, Minnesota
• Peer Reviewed:
  True
• Source Full Name:
  American Industrial Hygiene Association Journal
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:87f13372267680fab2146e68aba88bc83eb6e33a4305dbcd12217dc60efdfb045da81fd5b0658460eadceda77b6247b36ceb96637327fb15ab5896904e858fe8
• Download URL:
  https://stacks.cdc.gov/view/cdc/195737/cdc_195737_DS1.pdf
• File Type:
  [PDF - 405.83 KB ]