Cross platform lipid imaging of silver nanoparticle-exposed rat brain by LAESI-MS and MALDI-MS

Details

• Personal Author:
  Angel P ; Cantrell P ; Moss K ; Roberts, Jennifer R. ; Seeley EH ; Sriram K ; Walsh C
• Description:
  Overview: LAESI and MALDI imaging performed on brain samples to compare lipids detected. Lipid species detected highlight complementarity of techniques. Effects of silver nanoparticle exposure were evaluated using imaging modalities. Silver nanoparticles (AgNP) are widely used in industrial, household, diagnostic and antimicrobial products. Emerging studies suggest that AgNP can biodistribute to the brain following inhalation and potentially be neurotoxic. However, the molecular effects of AgNP exposure have not been fully explored. Here, we report the utility of differing MSI platforms in identifying rat brain lipidome changes following AgNP inhalation. The use of multiple mass spectrometry imaging (MSI) platforms has the advantage of both increasing and cross-validating the information gathered from individual MSI experiments. Lipid data are generated by classical matrixassisted laser desorption/ionization (MALDI) and laser ablation electrospray ionization (LAESI) MSI platforms and illustrate complementary signatures between platforms.These data increase our understanding of possible health effects due to AgNP in common products. Methods: Male Sprague-Dawley rats were exposed by inhalation to 1 mg/m3 AgNP (20 nm diameter, 0.3% wt/wt PVP coating) or filtered air (control) for 14 week days (4 h/d) and sacrificed 28 d post exposure. Brains were cryopreserved and sectioned. For MALDI, 12 um sections were washed, spray-coated with DHB or DAN using SunCollect (SunChrom), imaged by MALDI TOF (ultrafleXtreme, Bruker), and processed by flexImaging (Bruker). For LAESI, 30 um sections were washed, analyzed using LAESI DP-1000 coupled to an ion mobility mass spectrometer (Synapt G2 S, Waters) and the image data was normalized using ProteaPlot (Protea Biosciences). All imaging was performed in negative ion mode. Lipids were identified by accurate mass, MS/MS, and/or collision crosssections compared to available databases. [Description provided by NIOSH]
• Subjects:
  Animals Laboratories Lipids Metals Nervous System Occupational Health Respiratory System Safety Silver Toxicology
• Keywords:
  Analytical-processes Author Keywords: Nanotechnology Exposure-levels Inhalation Laboratory-animals Molecular-biology Nanotechnology Neurotoxic-effects Neurotoxicity Neurotoxicology Silver-compounds Workers

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• Publisher:
  American Society for Mass Spectrometry
• Document Type:
  Text
• Genre:
  Proceedings
• Place as Subject:
  Missouri ; OSHA Region 3 ; OSHA Region 7 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• NIOSHTIC Number:
  nn:20046856
• Citation:
  Proceedings of the 63rd ASMS Conference on Mass Spectrometry & Allied Topics, May 31-June 4, 2015, St. Louis, Missouri. Santa Fe, NM: American Society for Mass Spectrometry, 2015 May; :676
• CAS Registry Number:
  Silver (CAS RN 7440-22-4)
• Federal Fiscal Year:
  2015
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  Proceedings of the 63rd ASMS Conference on Mass Spectrometry & Allied Topics, May 31-June 4, 2015, St. Louis, Missouri
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:98907bd640d0971826f2ffc354497486c2f849feb17885e3708da93eb2f46595d6643ad604c8cae6d89e16b8fed0b808841c0972239b62728ab67ca75dfba191
• Download URL:
  https://stacks.cdc.gov/view/cdc/202137/cdc_202137_DS1.pdf
• File Type:
  [PDF - 793.65 KB ]