Proteomic analysis in the lung and brain of rats following silver nanoparticle inhalation

Details

• Personal Author:
  Battelli LA ; Boyce G ; Roberts, Jennifer R. ; Seeley EH ; Sriram K
• Description:
  Silver nanoparticles (AgNP) are widely used in industrial, household and diagnostic products, as well as in antimicrobial applications. Due to its high production volume there is potential for occupational and environmental exposure. Here, we investigated the potential toxic effects associated with AgNP both in the lung and brain. Male Sprague-Dawley rats were exposed by inhalation to 1 mg/m3 AgNPs (20 nm diameter, 0.3% PVP coated), or filtered air (control), for 4 h/d for 14 work days. At 1 and 28 d post-exposure, rats were humanely sacrificed, perfused with saline, and lungs and brains were harvested and sectioned for histology-guided mass spectrometry to determine localization of elemental silver in tissues and associated alterations in protein profiles. Digital microscopy images of the stained sections were annotated. After merging annotated and serial unstained section images, protein and metal ion mass spectra were collected from the annotated areas. Data were analyzed for differences between AgNP treated animals and controls. Focal areas in airways and parenchyma were analyzed from the lung. Several proteins were found to be differentially expressed at 1 and 28 d post-exposure. A peak consistent with thymosin beta4 was decreased and calcyclin increased in lungs of AgNP-exposed animals. Minimal changes were observed in brain cortex; however, significant differences in protein expression were observed in the striatum and hippocampus at 28 d after AgNP exposure. Specifically, in the hippocampus, myelin basic protein (14.1 kDa) was greatly elevated. Using matrix free laser desorption ionization, a signal consistent with elemental silver was detected in the lung but not the brain at 1 and 28 d post-exposure. Taken together, our findings suggest persistent alterations in both the lung and brain proteome following AgNP exposure, warranting further investigation of AgNP-related toxicity to avert or reduce potential human health risks. [Description provided by NIOSH]
• Subjects:
  Environmental Monitoring Hazardous Substances Histology Laboratories Lung Diseases Mass Spectrometry Microbiology Nervous System Nervous System Diseases Occupational Health Respiratory System Safety Silver Toxicology

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• Keywords:
  Author Keywords: Nanotechnology Brain-disorders Cell-alteration Cell-differentiation Dose-response Exposure-assessment Exposure-levels Inhalation-studies Laboratory-animals Laboratory-testing Lung-disorders Mass-spectrometry Mass Spectrometry Imaging Metallic-ions Microscopic-analysis Nanotechnology Neurotoxic-effects Neurotoxicity Protein-biochemistry Proteins Silver-compounds Tissue-culture Toxic-effects

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  California ; OSHA Region 3 ; OSHA Region 9 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  37
• Volume:
  144
• Issue:
  1
• NIOSHTIC Number:
  nn:20046045
• Citation:
  Toxicologist 2015 Mar; 144(1)(Abstract Suppl):37
• CAS Registry Number:
  Silver (CAS RN 7440-22-4)
• Federal Fiscal Year:
  2015
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 54th Annual Meeting and ToxExpo, March 22-26, 2015, San Diego, California
• Supplement:
  Abstract Suppl
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:aef80310d4abdd812fe874fe409983d51baa3a763576e9abaa8b6096f524afd7ab41325487e64ce47f42e2c64b16ce5fd9757d62598701ce06af8bc32476e53c
• Download URL:
  https://stacks.cdc.gov/view/cdc/201229/cdc_201229_DS1.pdf
• File Type:
  [PDF - 3.17 MB ]