Age impacts pulmonary inflammation and systemic bone response to inhaled organic dust exposure

Details

• Personal Author:
  Bailey KL ; Duryee MJ ; Dusad A ; Klassen LW ; Mikuls TR ; Poole JA ; Rombergerr DJ ; Staab E ; Thlele GM ; VanDeGraff J ; Wang D ; West WW ; Wyatt TA
• Description:
  Agricultural workers have high rates of airway and skeletal health disease. Studies recently demonstrated that inhaled agricultural organic dust extract (ODE)-induced airway injury is associated with bone deterioration in an animal model. However, the effect of age in governing these responses to organic dusts is unclear, but might be important in future approaches. Young (7-9 wk) and older (1214,o) male C57BL/6 mice received intranasal (i.n.) inhalation exposure to ODE from swine confinement facilities once or daily for 3 wk. Acute ODE-induced neutrophil influx and cytokine and chemokine (tumor necrosis factor [TNF]-alpha, interleukin [IL]-6, keratinocyte chemoattractant [CXCL1], macrophage inflammatory protein-2 [CXCL2]) airway production were reduced in older compared to young mice. Repetitive ODE treatment, however, increased lymphocyte recruitment and alveolar compartment histopathologic inflammatory changes in older mice. Whole lung cell infiltrate analysis revealed that young, but not older, mice repetitively treated with ODE demonstrated an elevated CD4:CD8 lymphocyte response. Acute inhalant ODE exposure resulted in a 4-fold and 1.5-fold rise in blood neutrophils in young and older mice, respectively. Serum IL-6 and CXCL1 levels were elevated in young and older mice i.n. exposed once to ODE, with increased CXCL1 levels in younger compared to older mice. Although older mice displayed reduced bone measurements compared to younger mice, younger rodents demonstrated ODE-induced decrease in bone mineral density, bone volume, and bone microarchitecture quality as determined by computed tomography (CT) analysis. Collectively, age impacts the airway injury and systemic inflammatory and bone loss response to inhalant ODE, suggesting an altered and enhanced immunologic response in younger as compared to older counterparts. [Description provided by NIOSH]
• Subjects:
  Age Factors Agriculture Dust Farmers Immune System Laboratories Lung Diseases Musculoskeletal Diseases Musculoskeletal System Occupational Health Safety

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• Keywords:
  Age-factors Agricultural-workers Bone-disorders Bone Disorders Immune-reaction Immunology Laboratory-animals Lung-disorders Organic-dusts
• ISSN:
  1528-7394
• Document Type:
  Text
• Funding:
  Cooperative Agreement ; Grant
• Genre:
  Journal Article
• Place as Subject:
  Nebraska ; OSHA Region 7
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  78
• Issue:
  19
• NIOSHTIC Number:
  nn:20046829
• Citation:
  J Toxicol Environ Health A 2015 Oct; 78(19):1201-1216
• Contact Point Address:
  Jill A. Poole, MD, Pulmonary, Critical Care, Sleep & Allergy Division; Department of Medicine, University of Nebraska Medical Center, 985990 The Nebraska Medical Center, Omaha, NE 68198-5990
• Email:
  japoole@unmc.edu
• Federal Fiscal Year:
  2016
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of Nebraska Medical Center - Omaha
• Peer Reviewed:
  True
• Start Date:
  20110901
• Source Full Name:
  Journal of Toxicology and Environmental Health, Part A: Current Issues
• End Date:
  20270831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:fd18abccc0a227f7eaba9f2deb5edc42b3b102f7715ac03013643f26dbb5cd4dbf03fb876522f1fb473a5b2dd7cc264c2995642f5b2f53b391252c8125ef474b
• Download URL:
  https://stacks.cdc.gov/view/cdc/202123/cdc_202123_DS1.pdf
• File Type:
  [PDF - 1.26 MB ]