Next day determination of ejaculatory sperm motility after overnight shipment of semen to remote locations

Details

• Personal Author:
  Bennett D ; Huszar G ; Janes M ; Sati L
• Description:
  PURPOSE: To develop a method for delayed assessment of sperm motility, after shipment of semen to a remote laboratory. Sperm in semen were labeled with the MitoTracker Red CM-H(2)XRos reagent, and fixed with 3.7 % formaldehyde by the laboratory technicians at the origin of the semen. This treatment reflected well sperm mitochondrial activity, and the MitoTracker signal was related to sperm motility and velocity for 2-3 days following ejaculation. METHODS: Sperm motility and velocity were evaluated manually and by computer assisted semen analysis (CASA), respectively. Fluorescence assessment of individual sperm was carried out with the computer assisted Metamorph v4.6.9 program. Emission levels of MitoTracker spermatozoa were studied in room temperature and cooled semen, or in the respective room temperature swim-up sperm fractions following ejaculation, and on the second day (N=103 samples, 89 men) and third day (N=10 samples, 8 men). RESULTS: Sperm with optical density (O.D.) =0.7 showed close correlations with ejaculatory sperm motility and velocity even after second day (r=0.92, p<0.001, N=103 samples). Further, the multiple of sperm motility and velocity was also related to the proportion of high MitoTracker reagent emission sperm (r=0.83, p<0.001, N=103 samples). MitoTracker dye fluorescence on the second day accurately reflected the ejaculatory sperm motility (r=0.90, p<0.001). Thus, a shipping delay would not adversely affect the results. CONCLUSIONS: The delayed assessment of sperm motility in samples treated with MitoTracker Red CM-H(2)XRos reagent and shipped to remote laboratory truly reflects the level of sperm motility at the time of the ejaculation. [Description provided by NIOSH]
• Subjects:
  Energy Metabolism Formaldehyde Laboratories Men Occupational Health Safety
• Keywords:
  Author Keywords: Sperm Mitochondrial Activity Formaldehydes Humans Metabolism Mito Tracker Physiology Shipped Semen Spermatozoa Sperm Velocity
• ISSN:
  1058-0468
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Connecticut ; Massachusetts ; OSHA Region 1
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  117-125
• Volume:
  32
• Issue:
  1
• NIOSHTIC Number:
  nn:20046791
• Citation:
  J Assist Reprod Genet 2015 Jan; 32(1):117-125
• Contact Point Address:
  Leyla Sati, Department of Histology and Embryology, Akdeniz University School of Medicine, Campus, 07070, Antalya, Turkey
• Email:
  leylasati@yahoo.com
• Federal Fiscal Year:
  2015
• Performing Organization:
  Yale University
• Peer Reviewed:
  True
• Start Date:
  19990930
• Source Full Name:
  Journal of Assisted Reproduction and Genetics
• End Date:
  20030929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:8d401771b8becc6acf4589900910056de0035186318c7def66c291515b46ac690d91deb275542d613bb15178032212fa07166359b05ce401ef0e098e1f54bbaa
• Download URL:
  https://stacks.cdc.gov/view/cdc/202105/cdc_202105_DS1.pdf
• File Type:
  [PDF - 4.95 MB ]