The pattern of tyrosine phosphorylation in human sperm in response to binding to zona pellucida or hyaluronic acid

Details

• Personal Author:
  Cayli S ; Delpiano E ; Huszar G ; Sakkas D ; Sati L
• Description:
  In mammalian species, acquisition of sperm fertilization competence is dependent on the phenomenon of sperm capacitation. One of the key elements of capacitation is protein tyrosine phosphorylation (TP) in various sperm membrane regions. In previous studies performed, the pattern of TP was examined in human sperm bound to zona pellucida of oocytes. In the present comparative study, TP patterns upon sperm binding to the zona pellucida or hyaluronic acid (HA) were investigated in spermatozoa arising from the same semen samples. Tyrosine phosphorylation, visualized by immunofluorescence, was localized within the acrosomal cap, equatorial head region, neck, and the principal piece. Tyrosine phosphorylation has increased in a time-related manner as capacitation progressed, and the phosphorylation pattern was identical within the principal piece and neck, regardless of the sperm bound to the zona pellucida or HA. Thus, the data demonstrated that the patterns of sperm activation-related TP were similar regardless of the spermatozoa bound to zona pellucida or HA. Further, sperm with incomplete development, as detected by excess cytoplasmic retention, failed to exhibit TP. [Description provided by NIOSH]
• Subjects:
  Energy Metabolism Men Occupational Health Safety
• Keywords:
  Author Keywords: Capacitation Humans Human Sperm Hyaluronic Acid Metabolism Phosphotyrosine Physiology Spermatozoa Zona Pellucida
• ISSN:
  1933-7191
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Connecticut ; Massachusetts ; OSHA Region 1
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  21
• Issue:
  5
• NIOSHTIC Number:
  nn:20046083
• Citation:
  Reprod Sci 2014 May; 21(5):573-581
• Contact Point Address:
  Gabor Huszar, Sperm Physiology Laboratory, Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06510
• Email:
  gabor.huszar@yale.edu
• Federal Fiscal Year:
  2014
• Performing Organization:
  Yale University
• Peer Reviewed:
  True
• Start Date:
  19990930
• Source Full Name:
  Reproductive Sciences
• End Date:
  20030929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:8b666cdf5c9cd6209f1e659596b0d63baef857e04385445e66632eb9a78aab74a44c7d34cf827c3ca063d2f666b5b98a017c0febe830bb297abc6974e77b0a39
• Download URL:
  https://stacks.cdc.gov/view/cdc/201255/cdc_201255_DS1.pdf
• File Type:
  [PDF - 408.38 KB ]