Multi-walled carbon nanotubes activate macrophages and NF-KB signaling to elicit inflammatory and fibrotic responses in mouse lungs

Details

• Personal Author:
  Dong J ; Ma Q
• Description:
  Macrophages play multiple roles in the pulmonary response to deposition of foreign bodies, such as particles, fibers. and microbes. in the lungs. As Ihe major first line of defenders, macrophages phagocytize and kill in the case of microorganisms, or transport for clearance in the case of particles and fibers. Macrophage, also secrete numerous soluble mediators to stimulate inflammatory and fibrotic responses 10 facilitate clearance and tissue repair, Under pathologic conditions, the responses become exacerbated and pulmonary fibrosis takes place, causing excessive deposition of collagen fibers and destruction of lung structures. Carbon nanotubes (CNT) are new nanomaterials made of one-atom-tltick carbon nanotubes in either a single layer (SWCNT) or concentric multiple layers (MWCNT). CNT have been developed for a wide range of industrial and commercial applications. However. their small size and fiber-like shape make them respirable and potentially pathogenic, causing adverse effects in the lungs Understanding the health effects of exposure to CNT has become increasingly important in nano safety. We found that MWCNT administered to mice by pharyngeal aspiration elicited signif,cant inflammatory and fibrotic pathology in lhe lungs, The early phase response was characterized by prominent infammatory infiltration. elevated levels of proinflammatory cytokines in the BAL fluid and lung parenchyma, and increased deposition of collagen fibers in the interstitial space, Macrophages were a dominant cell type during inflammatory infiltration in the early phase response and for the formation of granulomas and chronic fibrosis. In cultured macrophages. MWCNT directly stimulated secretion of proinflammatory and profibrotic cytokines and growth factors, and activation of the NF-kB pathway. Our study provided direct evidence for a critical role of lung macrophages in the development of pulmonary fibrosis following exposure to carbon nanotubes. [Description provided by NIOSH]
• Subjects:
  Animals Fibrosis Laboratories Lung Microbiology Occupational Health Particulate Matter Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Fibrous-bodies Laboratory-animals Lung-cells Lung-fibrosis Lung-tissue Microorganisms Nanotechnology Particulates Pulmonary-system Pulmonary-system-disorders Respiration Respiratory-system-disorders

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• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  New Hampshire ; OSHA Region 1 ; OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  1
• NIOSHTIC Number:
  nn:20046734
• Citation:
  The Behaviour and Function of Phagocytes in Health and Disease, May 31-June 5, 2015, Waterville Valley, New Hampshire. Waterville Valley, NH: Gordon Research Conference, 2015 May; :1
• Contact Point Address:
  Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, 1095 Willowdale Road, M
• Federal Fiscal Year:
  2015
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  The Behaviour and Function of Phagocytes in Health and Disease, May 31-June 5, 2015, Waterville Valley, New Hampshire
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:02dfda48413d023fc506616638c5a1507aca427b1902deff63ab71cd048b67502bf4430dca05c6d8ad648eeba087278ec1961cc7c88a1120f40a12f71083da79
• Download URL:
  https://stacks.cdc.gov/view/cdc/202068/cdc_202068_DS1.pdf
• File Type:
  [PDF - 787.15 KB ]