Development of phage immuno-loop-mediated isothermal amplification assays for organophosphorus pesticides in agro-products

Details

• Personal Author:
  Gee SJ ; Hammock BD ; Hua X ; Kim HJ ; Li M ; Liu F ; Shi H ; Wang H ; Wang M ; Wang Y ; Ye Y ; Yin W
• Description:
  Two immuno-loop-mediated isothermal amplification assays (iLAMP) were developed by using a phage-borne peptide that was isolated from a cyclic eight-peptide phage library. One assay was used to screen eight organophosphorus (OP) pesticides with limits of detection (LOD) between 2 and 128 ng mL(-1). The iLAMP consisted of the competitive immuno-reaction coupled to the LAMP reaction for detection. This method provides positive results in the visual color of violet, while a negative response results in a sky blue color; therefore, the iLAMP allows one to rapidly detect analytes in yes or no fashion. We validated the iLAMP by detecting parathion-methyl, parathion, and fenitrothion in Chinese cabbage, apple, and greengrocery, and the detection results were consistent with the enzyme-linked immunosorbent assay (ELISA). In conclusion, the iLAMP is a simple, rapid, sensitive, and economical method for detecting OP pesticide residues in agro-products with no instrumental requirement. [Description provided by NIOSH]
• Subjects:
  Agriculture Chemistry Environmental Exposure Enzymes Insecticides Occupational Health Organophosphates Safety
• Keywords:
  Agricultural-industry Analytical-processes Chemical-composition Chemical-properties Environmental-exposure Exposure-levels Foodstuff Organo-phosphorus-pesticides Peptides Pesticides
• ISSN:
  0003-2700
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  86
• Issue:
  16
• NIOSHTIC Number:
  nn:20046654
• Citation:
  Anal Chem 2014 Aug; 86(16):8441-8447
• Email:
  wangmha@njau.edu.cn
• Federal Fiscal Year:
  2014
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of California - Davis
• Peer Reviewed:
  True
• Start Date:
  20010930
• Source Full Name:
  Analytical Chemistry
• End Date:
  20270929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:ebb3c22cb74c3ec2d16b623f0516ff5f56b442f78f569543796d9c7c678bfe20e5866aa9b7f8bdf58f5c46fc319dd721a2fffe5fe55496b87af65f610340e4fe
• Download URL:
  https://stacks.cdc.gov/view/cdc/202015/cdc_202015_DS1.pdf
• File Type:
  [PDF - 2.17 MB ]