Association of virus content with its carrying particle size

Details

• Personal Author:
  Appert J ; Chander Y ; Ge S ; Goyal S ; Kuehn T ; Kumar S ; Pui D ; Raynor P ; Zuo Z
• Description:
  Airborne transmission of viral diseases heavily depends on the size of the virus-carrying particles, since particle size governs many aspects such as the transport of virus aerosol in air, deposition within the human respiratory tract, and control by filtration. A primary mode in the submicron size range has been found for the size distribution of human expiratory particles. However, how the virus is distributed among particles of different sizes is not well understood. The objectives of this laboratory study were to quantify virus content as a function of particle size and to determine virus survivability in the airborne state. Five surrogate viruses (MS2 bacteriophage, human adenovirus, transmissible gastroenteritis coronavirus, swine influenza virus, and avian influenza virus) were aerosolized, size-selected (100-450 nm) using a differential mobility analyzer, and collected onto gelatin filters. Uranine dye was also nebulized with the virus, serving as a chemical tracer. The resulting particle size distribution was determined using a scanning mobility particle sizer. The collected samples were analyzed by infectivity assays, quantitative PCR, and spectrofluorometry. Results using MS2 bacteriophage suggest that the viable and total virus distributions are better represented by the particle volume rather than the number distribution. The amount of virus per particle increased with particle size, which could be described by a power-law relationship. However, the survivability of airborne virus was not significantly affected by particle size. These results will help better understand disease transmission by virus aerosols. Experiments are ongoing with the four animal viruses and the comparison of the five viruses is presented. [Description provided by NIOSH]
• Subjects:
  Aerosols Air Pollutants, Occupational Communicable Diseases Men Occupational Health Particulate Matter Public Health Respiratory System Safety Viral Diseases Women

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• Keywords:
  Aerosol-particles Airborne-particles Humans Infectious-diseases Public-health Respiration Viral-diseases Viral-infections
• Publisher:
  American Association for Aerosol Research
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Abstract or Summary
• Place as Subject:
  Minnesota ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• NIOSHTIC Number:
  nn:20046490
• Citation:
  Proceedings of the AAAR 31st Annual Conference, October 8-12, 2012, Minneapolis, Minnesota. Mount Laurel, NJ: American Association for Aerosol Research, 2012 Oct; :8HA.6
• Federal Fiscal Year:
  2013
• Performing Organization:
  University of Minnesota, Schools of Veterinary Medicine, Minneapolis, Minnesota
• Peer Reviewed:
  False
• Start Date:
  20080901
• Source Full Name:
  Proceedings of the AAAR 31st Annual Conference, October 8-12, 2012, Minneapolis, Minnesota
• End Date:
  20120831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:3b2fde545ad29560123381eb38860f809fd3b0020305ea306519873d59039f0a4c0d3967c05bb29faa6ac794b3752c55240f30216ffdc9b75d81177d4b9d05e1
• Download URL:
  https://stacks.cdc.gov/view/cdc/201538/cdc_201538_DS1.pdf
• File Type:
  [PDF - 165.33 KB ]