Importance of nanoparticle agglomeration on inflammation

Details

• Personal Author:
  Castranova, Vincent ; Holian A ; Porter DW ; Sager TM ; Wolfarth M
• Description:
  Suspension of nanoparticles into biological fluids results in agglomeration, producing variability into the results, as the actual dose of nanoparticles no longer corresponds with the particle number. Therefore, it is important to determine how the agglomeration state of the nanoparticles affects their interaction with cells and animals. This study evaluates the effect of different dispersing media on the agglomeration state of nanoparticles, and in parallel measures their interactions in vivo, to better understand the importance of interactions when particles are well dispersed vs. agglomerated. The current study evaluated the role of NLRP3 inflammasome activation in the pulmonary bioactivity of nano-sized nickel-oxide (NiO) subjected to differing dispersion methods. Current evidence suggests that macrophage engulfed nanoparticles can cause lysosomal membrane permeability and release of cathepsin-B, thus activating the NLRP3 inflammasome. In order to test the role of agglomeration state in activation of the NLRP3 inflammasome, NiO was suspended in four different dispersion media (phosphate buffered saline, dispersion medium (DM), Survanta, and Pluronics). Well and poorly-dispersed (sonicated at 25W continuous output, 20 min or 5 min, respectively) suspensions were created. Mice (male, C57BL/6J, 7 weeks old) were given 0-80 microg/mouse of NiO in the different states of dispersion via pharyngeal aspiration. At one day post-exposure, mice underwent whole lung lavage to collect samples for cytokine analysis. The results showed that pre-exposure dispersion status correlated with inflammasome activation. The sonication time/media combination that produced the smallest hydrodynamic particle size (NiO suspended in DM and sonicated for 20 minutes) produced a greatest increase in cathepsin-B release, as well as IL-1beta, and IL-18 release, compared to other sonication/media combinations. These results indicate that a greater degree of pre-exposure dispersion increases cathepsin-B release and, thus, promotes inflammasome activation. [Description provided by NIOSH]
• Subjects:
  Biological Products Body Fluids Cells, Cultured Environmental Monitoring Enzymes Immune System Laboratories Lung Occupational Health Particulate Matter Respiratory System Safety Toxicology Vibration

  More +
• Keywords:
  Analytical-processes Bioactivation Biological-effects Biological-material Body-fluids Cell-culture-techniques Dispersion Exposure-assessment Exposure-levels Hydrodynamics Immune-reaction Immune-system In-vivo-study Laboratory-animals Nanotechnology Particulates Proteins Pulmonary-function Pulmonary-system Ultrasonic-testing Ultrasonics

  More +
• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  California ; Montana ; OSHA Region 3 ; OSHA Region 8 ; OSHA Region 9 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  144
• Issue:
  1
• NIOSHTIC Number:
  nn:20046195
• Citation:
  Toxicologist 2015 Mar; 144(1)(Abstract Suppl):121
• CAS Registry Number:
  Nickel monoxide (CAS RN 1313-99-1)
• Federal Fiscal Year:
  2015
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 54th Annual Meeting and ToxExpo, March 22-26, 2015, San Diego, California
• Supplement:
  Abstract Suppl
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:f01e8b35e5324aece99abd92086de90a7a631e3a73e41fbba5fd6ec38158f0fad6b0bf44ea141adb111515bc6e838f2119966ee98107c4a349a81727b2114233
• Download URL:
  https://stacks.cdc.gov/view/cdc/201320/cdc_201320_DS1.pdf
• File Type:
  [PDF - 3.17 MB ]