Graphene oxide augments airway remodeling and hyperresponsiveness in a murine model of asthma

Details

• Personal Author:
  Fadeel B ; Gutkin DW ; Kagan VE ; Kisin ER ; Reynolds J ; Savolainen KM ; Shurin M ; Shvedova AA ; Star A ; Yanamala N
• Description:
  Growing evidence indicates that local inflammatory responses following nanoparticle (NP) exposure result in modified innate immune responses, facilitating the development of respiratory diseases including asthma. Graphene oxide (GO) - having unique physico-chemical properties - is a promising carbonaceous nanomaterial with a multitude of medical and industrial applications. Here we investigated the modulation of allergic response in a murine model of ovalbumin (OVA)-induced asthma by pulmonary exposure to GO. The data showed that GO administration at the time of initial allergen sensitization augmented airway hyperresponsiveness and airway remodeling in the form of goblet cell hyperplasia and smooth muscle hypertrophy. The levels of IL-4, IL-5 and IL-13 were reduced in bronchoalveolar lavage (BAL) fluid and serum of GO-treated mice as compared to OVA-only group. Exposure to GO reduced eosinophil accumulation, but increased alveolar macrophages and lymphocytes in BAL fluid, as compared to those in OVA-only treated animals. Similarly, GO administration during sensitization stimulated the production of OVA-specific IgG2a and down-regulated the levels of IgE and IgG1. Moreover, exposure to GO increased the macrophage production of the mammalian chitinases, CHI3L1 and AMCase, whose expression is associated with asthma. Conclusively, while GO exposure reduces Th2 immune response in a murine model of asthma, it potentiates airway remodeling and hyperresponsiveness. [Description provided by NIOSH]
• Subjects:
  Asthma Asthma, Occupational Blood Environmental Monitoring Hypersensitivity Immune System Laboratories Lung Occupational Health Respiratory System Respiratory Tract Diseases Safety Toxicology

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• Keywords:
  Allergens Allergic-reactions Alveolar-cells Bronchial-asthma Carbon-compounds Exposure-assessment Immune-reaction Laboratory-animals Laboratory-testing Lymphocytes Nanotechnology Oxides Proteins Pulmonary-function Pulmonary-system-disorders Sensitization

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  California ; OSHA Region 3 ; OSHA Region 9 ; Pennsylvania ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  144
• Issue:
  1
• NIOSHTIC Number:
  nn:20045894
• Citation:
  Toxicologist 2015 Mar; 144(1):141
• CAS Registry Number:
  Graphene (CAS RN 1034343-98-0)
• Federal Fiscal Year:
  2015
• NORA Priority Area:
  Mining
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 54th Annual Meeting and ToxExpo, March 22-26, 2015, San Diego, California
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:29695a5c715e7627ac1a834cb7fba33696aa2ef66b6f70cf3202aa9e19dc663a881bb8306275f1ea82fe4002ecd968dbc769cda02c4443a42ec2062cad284b7f
• Download URL:
  https://stacks.cdc.gov/view/cdc/201141/cdc_201141_DS1.pdf
• File Type:
  [PDF - 219.59 KB ]