Cadmium-induced ototoxicity in rat cochlear organotypic cultures

Details

• Personal Author:
  Ding D ; Jiang H ; Liu H ; Roth JA ; Salvi R ; Sun H ; Wu X
• Description:
  Cadmium (Cd), a widely used industrial metal, is extremely nephrotoxic and neurotoxic; however, its effects on the peripheral auditory system are poorly understood. To evaluate the ototoxicity of Cd, we treated cochlear organotypic cultures from postnatal day 3 rats with Cd concentrations from 10 to 500 microM for 24 or 48 h. Afterward, we evaluated the degree of damage to hair cells, auditory nerve fibers, and spiral ganglion neurons. Damage to the hair cells, auditory nerve fibers, and spiral ganglion neurons systematically increased in a dose and time-dependent manner. Exposure to Cd concentrations of 10 microM for 24 and 48 h resulted in minor inner and outer hair cell loss in the basal third of the cochlea. As Cd concentrations increased, toxicity spread toward the apex, also in a time-dependent manner. Treatment with 100 microM Cd for 48 h resulted in substantial (>30 %) hair cell loss over the entire cochlea. Cd was also toxic to auditory nerve fibers and spiral ganglion neurons; 100 microM of Cd for 24 h or 10 microM of Cd for 48 h resulted in considerable damage to auditory nerve fibers and spiral ganglion neurons. These findings are the first to demonstrate that Cd can cause significant lesions to peripheral auditory nerve fibers, spiral ganglion neurons, and sensory hair cells in organotypic cultures from postnatal cochleae. [Description provided by NIOSH]
• Subjects:
  Animals Cadmium Kidney Diseases Laboratories Metals Nervous System Occupational Health Pathology Safety Urogenital Diseases Urogenital System

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• Keywords:
  Auditory-system Author Keywords: Cadmium Cadmium-compounds Cell-function Cellular-function Drugs Hair Cells Laboratory-animals Metal-compounds Metallic-compounds Microscopy Nephrotoxins Nerve Fibers Neurotoxins Ototoxicity Spiral Ganglion Neurons

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• ISSN:
  1029-8428
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  New York ; OSHA Region 2
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  179-189
• Volume:
  26
• Issue:
  2
• NIOSHTIC Number:
  nn:20045750
• Citation:
  Neurotox Res 2014 Aug; 26(2):179-189
• Contact Point Address:
  Richard Salvi, Center for Hearing and Deafness, University at Buffalo, 137 Cary Hall, Buffalo, NY 14214, USA
• Email:
  salvi@buffalo.edu
• CAS Registry Number:
  Cadmium (CAS RN 7440-43-9)
• Federal Fiscal Year:
  2014
• NORA Priority Area:
  Manufacturing
• Performing Organization:
  State University of New York - Buffalo
• Peer Reviewed:
  True
• Start Date:
  20120901
• Source Full Name:
  Neurotoxicity Research
• End Date:
  20170831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:93a533cf1979d16fa65f58b5364392887d2dd11725be00f266033c6e35e48096b099331d06d86e9f596cadaee9e57319cf72bb9c938a446c6d9560e5c9a456d8
• Download URL:
  https://stacks.cdc.gov/view/cdc/201046/cdc_201046_DS1.pdf
• File Type:
  [PDF - 986.53 KB ]