Production of a Chaetomium globosum enolase monoclonal antibody

Details

• Personal Author:
  Beezhold, Donald H. ; Green, Brett J. ; Hettick JM ; Lemons, Angela R. ; Nayak, Ajay P. ; Rittenour WR
• Description:
  Chaetomium globosum is a hydrophilic fungal species and a contaminant of water-damaged building materials in North America. Methods to detect Chaetomium species include subjective identification of ascospores, viable culture, or molecular-based detection methods. In this study, we describe the production and initial characterization of a monoclonal antibody (MAb) for C. globosum enolase. MAb 1C7, a murine IgG1 isotype MAb, was produced and reacted with recombinant C. globosum enolase (rCgEno) in an enzyme-linked immunosorbent assay and with a putative C. globosum enolase in a Western blot. Epitope mapping showed MAb 1C7 specific reactivity to an enolase decapeptide, LTYEELANLY, that is highly conserved within the fungal class Sordariomycetes. Cross-reactivity studies showed MAb 1C7 reactivity to C. atrobrunneum but not C. indicum. MAb 1C7 did not react with enolase from Aspergillus fumigatus, which is divergent in only two amino acids within this epitope. The results of this study suggest potential utility of MAb 1C7 in Western blot applications for the detection of Chaetomium and other Sordariomycetes species. [Description provided by NIOSH]
• Subjects:
  Antibodies Construction Industry Construction Materials Fungi Occupational Health Safety
• Keywords:
  Antibody-response Bacteria Construction-materials Molds Molecular-biology
• ISSN:
  2167-9436
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  428-437
• Volume:
  33
• Issue:
  6
• NIOSHTIC Number:
  nn:20045555
• Citation:
  Monoclon Antib Immunodiagn Immunother 2014 Dec; 33(6):428-437
• Contact Point Address:
  B. J. Green, Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505
• Email:
  dox6@cdc.gov
• Federal Fiscal Year:
  2015
• NORA Priority Area:
  Services
• Peer Reviewed:
  True
• Source Full Name:
  Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:be3f7675a8378f3a15277ebf6bdcbc1e84d5e916c1523cfe0ed5568baa4f7ff7bdf457bbb630c6a6ace69c505d1f2a20686f85e8c4b414bab09c92e9bc08c3b5
• Download URL:
  https://stacks.cdc.gov/view/cdc/200931/cdc_200931_DS1.pdf
• File Type:
  [PDF - 593.79 KB ]