Lung epithelial cells resist influenza A infection by inducing the expression of cytochrome c oxidase VIc which is modulated by miRNA 4276

Details

• Personal Author:
  Beezhold, Donald H. ; Noti JD ; Othumpangat S
• Description:
  Influenza virus infection induces several changes in host miRNA profile, host cell death and tissue damage. Cytochrome c is a regulator of the intrinsic apoptotic pathway and is altered during viral infections. Within the first 3 h of infection with influenza virus, significant down-regulation of hsa-miRNA-4276 (miRNA-4276) is followed by a 2-fold increase in cytochrome c oxidase VIC (COX6C) mRNA was found to occur in human alveolar and bronchial epithelial cells. Expression of caspase-9 also increased within the first 3 h of infection, but subsequently decreased. Modulation of miR-4276 using mimic and inhibitor oligonucleotides showed significant down-regulation or up-regulation, respectively, of COX6C expression. Our data suggests that on initial exposure to influenza virus, host cells upregulate COX6C mRNA expression through silencing miR-4276 and repressed viral replication by inducing the apoptotic protein caspase-9. Taken together, these data suggest that miR-4276 may be an important regulator of the early stages of infection by influenza. [Description provided by NIOSH]
• Subjects:
  Cells, Cultured Cytology Genetics Immune System Lung Nucleotides Occupational Health Respiratory System Respiratory Tract Diseases Safety Viral Diseases

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• Keywords:
  Alveolar-cells Author Keywords: MiRNA Cell-alteration Cell-cultures Cell-damage Cell-function Cell-metabolism Cellular-reactions Cellular-respiration Cellular-transport-mechanism COX6C Cytopathology Genes Host-resistance Humans Immune-reaction Influenza Virus Lung-cells Lung Epithelial Cells Oxidative-phosphorylation Proteins Respiratory-infections Respiratory-system-disorders Ribonucleic-acids Viral-diseases Viral-infections

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• ISSN:
  0042-6822
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  256-264
• Volume:
  468
• NIOSHTIC Number:
  nn:20045072
• Citation:
  Virology 2014 Nov; 468-470:256-264
• Contact Point Address:
  Sreekumar Othumpangat, Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road, MS 4020, Morgantown, WV 26505, USA
• Federal Fiscal Year:
  2015
• NORA Priority Area:
  Healthcare and Social Assistance
• Peer Reviewed:
  True
• Source Full Name:
  Virology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:a925d7de5245adc31c078d14e1c8b00714385c70add96850a9517f55f376b2a4d03ea1f9a584f561a37cae2f9632adc6d0610522d8190d498d8fda9626855fd3
• Download URL:
  https://stacks.cdc.gov/view/cdc/200604/cdc_200604_DS1.pdf
• File Type:
  [PDF - 670.96 KB ]