The relationship between alveolar macrophage TNF, IL-1, and PGE2 release, alveolitis, and disease severity in sarcoidosis

Details

• Personal Author:
  Dayton CS ; Gilbert SR ; Hunninghake GW ; Pueringer RJ ; Schwartz DA
• Description:
  A mononuclear cell alveolitis, comprised in part of activated macrophages, is thought to precede granuloma formation and fibrosis in pulmonary sarcoidosis. Tumor necrosis factor-alpha (TNF), interleukin 1-beta (IL-1), and prostaglandin E2 (PGE2) are potent mediators released by activated alveolar macrophages. To determine if alveolar macrophage TNF, IL-1, and PGE2 release was associated with clinically progressive pulmonary sarcoidosis, we obtained alveolar macrophages from bronchoalveolar lavage of 68 patients with biopsy specimen-confirmed sarcoidosis, cultured the macrophages in the presence and absence of lipopolysaccharide (10 mg/L) for 24 h, and measured TNF (enzyme-linked immunosorbent assay), IL-1 (enzyme-linked immunosorbent assay), and PGE2 (radioimmunoassay) release. Alveolar macrophages from most patients with sarcoidosis spontaneously released TNF, IL-1, and PGE2. The amounts of these mediators released (either spontaneously or following lipopolysaccharide stimulation) did not positively correlate with the numbers of any of the cells in bronchoalveolar lavage fluid, the clinical status of disease (stable vs deterioration), steroid usage, or cigarette smoking. The relative release of each of the individual mediators, however, was highly correlated with the release of the other mediators. The studies suggest that these markers of alveolar macrophage activation from a single bronchoalveolar lavage are poor indicators of clinically progressive disease. [Description provided by NIOSH]
• Subjects:
  Cells, Cultured Epidemiology Fibrosis Immune System Lung Lung Diseases Men Occupational Health Respiratory System Respiratory Tract Diseases Safety Women

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• Keywords:
  Cell-cultures Cell-function Humans Immunology Lung-disease Lung-disorders Lung-fibrosis Pulmonary-disorders Pulmonary-function Pulmonary-system Pulmonary-system-disorders Respiratory-system-disorders

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• ISSN:
  0012-3692
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Iowa ; OSHA Region 7
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  103
• Issue:
  3
• NIOSHTIC Number:
  nn:20044963
• Citation:
  Chest 1993 Mar; 103(3):832-838
• Contact Point Address:
  Dr. David Schwurtz, Internal Medicine, Uniwsity of Iowa Hospitals and Clinics, Iowa City 52242-1081
• Federal Fiscal Year:
  1993
• Performing Organization:
  University of Iowa, Iowa City, Iowa
• Peer Reviewed:
  True
• Start Date:
  19900701
• Source Full Name:
  Chest
• End Date:
  19930630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:53df6b2ac3980180abf2acf4a99fcf15c17fffd91ac5ac0cff4299ea00d28596c368de4280da4c0235bd66a31d01d1d9e0ccc02da18fc05cb029bd1e622f1fd8
• Download URL:
  https://stacks.cdc.gov/view/cdc/200540/cdc_200540_DS1.pdf
• File Type:
  [PDF - 702.19 KB ]