Development of a sensitive monoclonal antibody-based enzyme-linked immunosorbent assay for the analysis of paclobutrazol residue in wheat kernel

Details

• Personal Author:
  Cao Z ; Cui Y ; Li QX ; Tan G ; Wang B ; Zhang L ; Zhao H
• Description:
  An indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed with monoclonal antibody (mAb) mAb6H73C9 recognizing the plant growth regulator paclobutrazol (PBZ). The icELISA had a half-maximum inhibition concentration (IC50) and working range of approximately 8.7 and 2.0-50.4 ng/mL, respectively. Average recoveries of PBZ in the wheat (Triticum aestivum) kernel samples were between 84.3 and 118.9% with relative standard deviations between 3.9 and 14.2%. As determined by the icELISA and further confirmed by liquid chromatography-electrospray ionization quadrupole Orbitrap mass spectrometry (LC-ESI-MS) analysis, the maximum residue concentration was about 0.07 mg/kg in the kernel samples, which indicated that PBZ could transfer from PBZ- treated seedlings to the kernel samples. The correlation coefficient (R2) between icELISA and LC-ESI-MS results was 0.979, which manifested that the developed icELISA was sensitive enough for monitoring PBZ residues in wheat kernels. [Description provided by NIOSH]
• Subjects:
  Agriculture Antibodies Biological Assay Electroplating Enzymes Food Contamination Insecticides Mass Spectrometry Occupational Health Safety
• Keywords:
  Agricultural-products Antibody-response Author Keywords: Paclobutrazol Bioassays Electrostatic-spraying ELISA Enzyme-activity Food-contaminants Immunological-tests Ionization LC-ESI-MS Liquid-chromatography Mass-spectrometry Monoclonal Antibody Pesticide-residues Plant-cells Plants Wheat Kernel

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• ISSN:
  0021-8561
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  California ; Hawaii ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  62
• Issue:
  8
• NIOSHTIC Number:
  nn:20044765
• Citation:
  J Agric Food Chem 2014 Feb; 62(8):1826-1831
• Contact Point Address:
  Guiyu Tan, College of Science, China Agricultural University, Beijing 100193, China
• Email:
  guiyu-0401@163.com
• CAS Registry Number:
  Paclobutrazol (CAS RN 76738-62-0)
• Federal Fiscal Year:
  2014
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of California - Davis
• Peer Reviewed:
  True
• Start Date:
  20010930
• Source Full Name:
  Journal of Agricultural and Food Chemistry
• End Date:
  20270929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:49d2b3949e8339544b82f4bf2e45520296ef00025db4578809db0f89ba69bbc128b48628a98f19761fa8a706d8b6cb7295aeddbb197f6928b639e128a4fa86fd
• Download URL:
  https://stacks.cdc.gov/view/cdc/200399/cdc_200399_DS1.pdf
• File Type:
  [PDF - 414.49 KB ]