Method for Simultaneous Measurement of Antibodies to 23 Pneumococcal Capsular Polysaccharides

Details

• Personal Author:
  Biagini, Raymond E. ; MacKenzie, Barbara A. ; Sammons DL ; Schlottmann SA ; Smith JP ; Snawder JC ; Striley CAF ; Weissman, David N.
• Description:
  We describe a fluorescent covalent microsphere immunoassay (FCMIA) method for the simultaneous (multiplexed) measurement of immunoglobulin G (IgG) antibodies to 23 pneumococcal capsular polysaccharide (PnPS) serotypes present in the pneumococcal polysaccharide vaccine (PPV23) licensed by the Food and Drug Administration, i.e., PnPSs 1, 2, 3, 4, 5, 6B, 7F, 8, 9N, 9V, 10A, 11A, 12F, 14, 15B, 17F, 18C, 19A, 19F, 20, 22F, 23F, and 33F. In addition, the assay incorporates an internal control that allows for contemporaneous evaluation of the effectiveness of pneumococcal cell wall polysaccharide (C-PS) preadsorption and a second control of PnPS 25 (which is not present in any polysaccharide or conjugate vaccine), which can be used to evaluate interassay reproducibility (useful for pre- versus postvaccination studies). The FCMIA was standardized with U.S. reference antipneumococcal serotype standard serum 89S-2. Preadsorption of 89S-2 with each PnPS and C-PS yielded homologous inhibition for serotypes 1, 6B, 9N, 9V, 11A, 12F,14, 15B, 18C, 19A, 19F, 20, 22F, 25, and 33F; heterologous inhibition for serotypes 9V, 10A, 11A, 12F, 15B, 17F, 20, and 23F; and neither homologous nor heterologous inhibition for serotypes 2, 3, 4, and 5. The minimum detectable concentrations for the 24 multiplexed (PnPS and C-PS) FCMIAs ranged from 20 pg/ml for PnPS 3 to 600 pg/ml for PnPS 14. The PnPS FCMIA method has numerous benefits over enzyme-linked immunosorbent assays commonly used to measure anti-PnPS-specific IgG levels, including increased speed, smaller sample volumes, equivalent or better sensitivity, and increased dynamic range. [Description provided by NIOSH]
• Subjects:
  Antibodies Biological Assay Enzymes Immune System Laboratories Occupational Health Safety
• Keywords:
  Antibody Response Bioassays Biological Monitoring Enzyme Activity Immunoglobulins Immunological Tests Immunotoxins Laboratory Testing Polysaccharides Proteins Sampling Serology Vaccines

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• ISSN:
  1071-412X
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 3 ; OSHA Region 5 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division ; DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  10
• Issue:
  5
• NIOSHTIC Number:
  nn:20023573
• Citation:
  Clin Diagn Lab Immunol 2003 Sep; 10(5):744-750
• Contact Point Address:
  Raymond E. Biagini, Division of Applied Research and Technology, National Institute for Occupational Safety and Health, MS-C26, 4676 Columbia Parkway, Cincinnati, OH 45226
• Email:
  reb4@cdc.gov
• Federal Fiscal Year:
  2003
• Peer Reviewed:
  True
• Source Full Name:
  Clinical and Diagnostic Laboratory Immunology
• End Date:
  20020930
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:d2ed072378fc254763a1d5dad8bfae3e329cf7ff99090edd41799c40b7ff45549ebcb035a69ac8a3b754fdf658c10f25cbb91d7f5938914b5c44359815d648b6
• Download URL:
  https://stacks.cdc.gov/view/cdc/195927/cdc_195927_DS1.pdf
• File Type:
  [PDF - 144.26 KB ]