Phospholipase A(2) activation by poultry particulate matter is mediated through extracellular signal-regulated kinase in lung epithelial cells: regulation of interleukin-8 release

Details

• Personal Author:
  Baran CP ; Kotha SR ; Malireddy S ; Marsh CB ; Nana-Sinkam PS ; Parinandi NL ; Patel RB ; Piper MG ; Romberger D ; Sliman S ; Wewers MD ; Zhao L
• Description:
  The mechanisms of poultry particulate matter (PM)-induced agricultural respiratory disorders are not thoroughly understood. Hence, it is hypothesized in this article that poultry PM induces the release of interleukin-8 (IL-8) by lung epithelial cells that is regulated upstream by the concerted action of cytosolic phospholipase A2 (cPLA2) and extracellular signal-regulated kinase (ERK). To test this hypothesis, the widely used cultured human lung epithelial cells (A549) were chosen as the model system. Poultry PM caused a significant activation of PLA2 in A549 cells, which was attenuated by AACOCF3 (cPLA2 inhibitor) and PD98059 (ERK-1/2 upstream inhibitor). Poultry PM induced upstream ERK-1/2 phosphorylation and downstream cPLA2 serine phosphorylation, in a concerted fashion, in cells with enhanced association of ERK-1/2 and cPLA2. The poultry PM-induced cPLA2 serine phosphorylation and IL-8 release were attenuated by AACOCF3, PD98059, and by transfection with dominant-negative ERK-1/2 DNA in cells. The poultry PM-induced IL-8 release by the bone marrow-derived macrophages of cPLA2 knockout mice was significantly lower. For the first time, this study demonstrated that the poultry PM-induced IL-8 secretion by human lung epithelial cells was regulated by cPLA2 activation through ERK-mediated serine phosphorylation, suggesting a mechanism of airway inflammation among poultry farm workers. [Description provided by NIOSH]
• Subjects:
  Agriculture Air Pollutants, Occupational Animal Husbandry Animals Cells, Cultured Dust Food Industry Immune System Lung Lung Diseases Occupational Health Particulate Matter Respiratory System Respiratory Tract Diseases Safety

  More +
• Keywords:
  A549 Cells Agricultural-industry Agricultural-workers Agricultural PM Airborne-dusts Airborne-particles Airway-obstruction Animal-husbandry-workers Author Keywords: Phospholipase A2 Cell-cultures Cellular-reactions CPLA2 Serine Phosphorylation Dust-inhalation Dust-particles ERK IL-8 Immune-reaction Inflammatory Cytokines Interleukin-8 Lipases Lung-cells Lung-disorders Lung Epithelial Cell MAPK Organic-dusts Particulate-dust Poultry Poultry-industry Poultry-workers Poultry Dust Pulmonary-system-disorders Respiratory-system-disorders Respiratory Epithelium

  More +
• ISSN:
  1085-9195
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Nebraska ; Ohio ; OSHA Region 5 ; OSHA Region 7
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  415-429
• Volume:
  67
• Issue:
  2
• NIOSHTIC Number:
  nn:20044279
• Citation:
  Cell Biochem Biophys 2013 Nov; 67(2):415-429
• Contact Point Address:
  Narasimham L Parinandi, Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, Department of Medicine, Dorothy M. Davis Heart and Lung Research Institute, College of Medicine, The Ohio State University, Columbus, OH 43210, USA
• Email:
  narasimham.parinandi@osumc.edu
• Federal Fiscal Year:
  2014
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of Nebraska Medical Center, Omaha, Nebraska
• Peer Reviewed:
  True
• Start Date:
  20060801
• Source Full Name:
  Cell Biochemistry and Biophysics
• End Date:
  20160731
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:1311f5ef554a29957d10a7a0b8d2ff13e9a9b640e7ba1e8c4e10643bbdc8732009e4ebd63c7206a8d1d2cc6068202f0ff7564943e47e5823d968b28a630af69e
• Download URL:
  https://stacks.cdc.gov/view/cdc/200017/cdc_200017_DS1.pdf
• File Type:
  [PDF - 1.05 MB ]