Airborne agricultural particulate matter induces inflammatory cytokine secretion by respiratory epithelial cells: mechanisms of regulation by eicosanoid lipid signal mediators

Details

• Personal Author:
  Hart J ; Kotha SR ; Lawson C ; Magalang UJ ; Malireddy S ; Marsh CB ; Parinandi NL ; Patel RB ; Romberger D ; Steinhour E ; Wewers MD ; Wilkins, John R. ; Zhao L
• Description:
  The purpose of this study was to elucidate the mechanism of the airborne poultry dust (particulate matter, PM)-induced respiratory tract inflammation, a common symptom in agricultural respiratory diseases. The study was based on the hypothesis that poultry PM would induce the release of inflammatory cytokine interleukin-8 (IL-8) by respiratory epithelial cells under the upstream regulation by cytosolic phospholipase A2 (cPLA2) activation and subsequent formation of cyclooxygenase (COX)- and lipoxygenase (LOX)-catalyzed arachidonic acid (AA) metabolites (eicosanoids). Human lung epithelial cells (A549) in culture were treated with the poultry PM (0.1-1.0 mg) for different lengths of time, following which PLA2 activity, release of eicosanoids and secretion of IL-8 in cells were determined. Poultry PM (1.0 mg/ml) caused a significant activation of PLA2 in a time-dependent manner (15-60 min), which was significantly attenuated by the calcium-chelating agents, cPLA2-specific inhibitor (AACOCF3) and antioxidant (vitamin C) in A549 cells. Poultry PM also significantly induced the release of COX- and LOX-catalyzed eicosanoids (prostaglandins, thromboxane A2 and leukotrienes B4 and C4) and upstream activation of AA LOX in the cells. Poultry PM also significantly induced release of IL-8 by the cells in a dose- and time-dependent manner, which was significantly attenuated by the calcium chelating agents, antioxidants and COX- and LOX-specific inhibitors. The current study for the first time revealed that the poultry PM-induced IL-8 release from the respiratory epithelial cells was regulated upstream by reactive oxygen species, cPLA2-, COX- and LOX-derived eicosanoid lipid signal mediators. [Description provided by NIOSH]
• Subjects:
  Agriculture Air Pollutants, Occupational Animal Husbandry Animals Antioxidants Cells, Cultured Chelating Agents Dust Farmers Food Industry Laboratories Lipids Lung Lung Diseases Occupational Health Particulate Matter Respiratory System Respiratory Tract Diseases Safety

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• Keywords:
  Agricultural-industry Agricultural-workers Airborne-dusts Airborne-particles Animal-husbandry-workers Author Keywords: Organic Poultry Dust Cell-cultures Chelating-agents Interleukin-8 Laboratory-testing Leukocytes Leukotrienes Lipases Lung-cells Lung-disorders Lung Epithelial Cell Occupational Farm Respiratory Diseases Organic-dusts Oxidative-processes Particulate-dust Phospholipase A2 Phospholipids Poultry Poultry-industry Poultry-workers Prostaglandin-inhibitors Prostaglandins Pulmonary-system-disorders Respiratory-system-disorders

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• ISSN:
  0301-1208
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Nebraska ; Ohio ; OSHA Region 5 ; OSHA Region 7
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  387-401
• Volume:
  50
• Issue:
  5
• NIOSHTIC Number:
  nn:20044255
• Citation:
  Indian J Biochem Biophys 2013 Oct; 50(5):387-401
• Contact Point Address:
  Narasimham L Parinandi, Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, Dorothy M. Davis Heart & Lung Research Institute, The Ohio State University, College of Medicine, Columbus, OH 43210 USA
• Email:
  narasimham.parinandi@osumc.edu
• Federal Fiscal Year:
  2014
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of Nebraska Medical Center, Omaha, Nebraska
• Peer Reviewed:
  True
• Start Date:
  20060801
• Source Full Name:
  Indian Journal of Biochemistry and Biophysics
• End Date:
  20160731
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:92a7af7e452f519dff47694ee7691a39374e52f62abc4ce8b49fa530d7fa93a422c0e2b06200a606c1020823848fca1a26662256bdd51ac0cab264e0b2f7d27f
• Download URL:
  https://stacks.cdc.gov/view/cdc/199999/cdc_199999_DS1.pdf
• File Type:
  [PDF - 493.34 KB ]