Alterations in cardiomyocyte function after pulmonary treatment with stainless steel welding fume in rats

Details

• Personal Author:
  Antonini JM ; Castranova, Vincent ; Fekedulegn DB ; Kan H ; Popstojanov R ; Salmen R ; Ye M ; Zheng W
• Description:
  Welding fume is composed of a complex of different metal particulates. Pulmonary exposure to different welding fumes may exert a negative impact on cardiac function, although the underlying mechanisms remain unclear. To explore the effect of welding fumes on cardiac function, Sprague-Dawley rats were exposed by intratracheal instillation to 2 mg/rat of manual metal arc hard surfacing welding fume (MMA-HS) once per week for 7 wk. Control rats received saline. Cardiomyocytes were isolated enzymatically at d 1 and 7 postexposure. Intracellular calcium ([Ca2+]i) transients (fluorescence ratio) were measured on the stage of an inverted phase-contrast microscope using a myocyte calcium imaging/cell length system. Phosphorylation levels of cardiac troponin I (cTnI) were determined by Western blot. The levels of nonspecific inflammatory marker C-reactive protein (CRP) and proinflammatory cytokine interleukin-6 (IL-6) in serum were measured by enzyme-linked immunosorbent assay (ELISA). Contraction of isolated cardiomyocytes was significantly reduced at d 1 and d 7 postexposure. Intracellular calcium levels were decreased in response to extracellular calcium stimulation at d 7 postexposure. Changes of intracellular calcium levels after isoprenaline hydrochloride (ISO) stimulation were not markedly different between groups at either time point. Phosphorylation levels of cTnI in the left ventricle were significantly lower at d 1 postexposure. The serum levels of CRP were not markedly different between groups at either time point. Serum levels of IL-6 were not detectable in both groups. Cardiomyocyte alterations observed after welding fume treatment were mainly due to alterations in intracellular calcium handling and phosphorylation levels of cTnI. [Description provided by NIOSH]
• Subjects:
  Air Pollutants, Occupational Animals Cardiovascular System Laboratories Lung Metals Occupational Health Respiratory System Respiratory Tract Diseases Risk Assessment Risk Factors Safety Welding

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• Keywords:
  Cardiac-function Exposure-levels Fumes Laboratory-animals Metal-fumes Metallic-compounds Metallic-fumes Pulmonary-function Pulmonary-system Pulmonary-system-disorders Risk-factors

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• ISSN:
  1528-7394
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 3 ; OSHA Region 9 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  77
• Issue:
  12
• NIOSHTIC Number:
  nn:20044220
• Citation:
  J Toxicol Environ Health A 2014 May; 77(12):705-715
• Contact Point Address:
  Risto Popstojanov, Health Effects Laboratory Division (HELD), National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505
• Email:
  popstojanov@yahoo.com
• Federal Fiscal Year:
  2014
• NORA Priority Area:
  Construction
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Toxicology and Environmental Health, Part A: Current Issues
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:0a5a560fec8bb749c497b3f17d23cd395c1c01c57f2c297cb9180359206c558c1481b833aa2652c1aa9b5280bd6217eead65e97870bfd59361301724a309068e
• Download URL:
  https://stacks.cdc.gov/view/cdc/199979/cdc_199979_DS1.pdf
• File Type:
  [PDF - 388.22 KB ]