Different functionalizations of MWCNT influence transformation potential in primary human lung epithelial cells

Details

• Personal Author:
  Castranova, Vincent ; Chen M ; Derk R ; Rojanasakul Y ; Stueckle, Todd A. ; Wang L
• Description:
  MWCNT have received increased scrutiny for potential human health impacts based on their fibrogenicity and promotion of pulmonary carcinogenesis. Functionalized CNT (fCNT) development has intensified to improve surface activity in technological applications, and potentially reduce toxicity. Subchronic in vitro CNT exposure causes neoplastic-like transformation; however, tumorigenic risk associated with fMWCNT exposure in human lung epithelium is presently unknown. To identify early steps in fMWCNT-induced cell transformation, this study hypothesized that different functional groups of MWCNT determine their neoplastic transformation potential in primary human small airway epithelial cells (SAECs). Cells were continuously exposed (0.06mg/cm2) to dispersed pure (pMWCNT), carboxylated (cMWCNT), and aminated MWCNT (nMWCNT) for 8 and 12 weeks. Dispersed ultrafine carbon black (UFCB) and crocidolite asbestos served as controls. Exposed cells were assessed for several established cancer hallmarks and morphological transformation. UFCB and pMWCNT cells at 48h and all MWCNT cells at 6d post-treatment exhibited significant increased proliferation compared to controls. UFCB exposure stimulated significant invasion and migration behavior while pMWCNT and cMWCNT showed moderate significant increases; however, these trends disappeared at 6d post-exposure. Conversely, nMWCNT displayed the largest significant increase in colony formation potential while UFCB showed a moderate significant increase. All other treatments did not differ from controls. UFCB and nMWCNT cells exhibited increased foci frequency, indicative of neoplastic transformation, compared to all other treatments. In summary, surface charge characteristics of carbon nanoparticles can impact transient, early neoplastic transformation events in vitro following occupationally relevant exposures. [Description provided by NIOSH]
• Subjects:
  Carcinogens Lung Lung Diseases Occupational Health Respiratory System Respiratory Tract Diseases Safety Toxicology
• Keywords:
  Carcinogenesis Cell-damage Cell-function Cellular-function Exposure-levels In-vitro-study Nanotechnology Pulmonary-disorders Pulmonary-function Pulmonary-system Pulmonary-system-disorders

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  138
• Issue:
  1
• NIOSHTIC Number:
  nn:20043888
• Citation:
  Toxicologist 2014 Mar; 138(1):154
• Federal Fiscal Year:
  2014
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 53rd Annual Meeting and ToxExpo, March 23-27, 2014, Phonex, Arizona
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:c0904e0e8bdea229759aaf96b6c451d2af5dfb5c5413f5ebca93830ef95abd1328a32bb6c7a571640236497524bfd56c862af82e403c69db9b6dc48da52de83c
• Download URL:
  https://stacks.cdc.gov/view/cdc/199761/cdc_199761_DS1.pdf
• File Type:
  [PDF - 246.76 KB ]