Effects of MWCNT and nitrogen-doped MWCNT in lung epithelial cells

Details

• Personal Author:
  Castranova, Vincent ; Endo M ; Mihalchik A ; Porter DW ; Qian Y ; Tsuruoka S
• Description:
  The growing use of multi-walled carbon nanotubes (MWCNT) and their derivatives in academic and industrial settings has raised the need to efficiently and accurately determine their potential toxicity in the workplace. Nitrogen-doped multi-walled carbon nanotubes (ND-MWCNT) are modified MWCNT that have enhanced electrical conductivity and are increasingly used in a variety of aerospace and fuel cell applications. Although similar in structure to MWCNT, limited toxicological data is available, and the biocompatibility and mechanism of action of ND-MWCNT have yet to be elucidated. Recent in vivo data showed that NDMWCNT induced inflammation and fibrosis in mouse lungs. In this study, we assess uptake of ND-MWCNT into small airway epithelial cells (SAEC), which may induce molecular toxicological effects. We showed that ND-MWCNT induced higher levels of reactive oxygen species (ROS) in SAEC when compared to their parent MWCNT in short-term in vitro exposure. Treatment of SAEC with low doses (1.2ug/mL) of ND-MWCNT and MWCNT indicated that both induced a significant increase in cell proliferation in a time-dependent manner. Furthermore, significant alterations to the cell cycle were observed in cells treated with both ND-MWCNT and MWCNT in a time- and dose-dependent manner, as shown by an increased percentage of cells in the S and G2 phases of the cell cycle. Confocal images showed alterations in acetylated and total tubulin levels in both ND-MWCNT and MWCNT treated cells as well. Since increases in ROS production and cellular proliferation are associated with multiple pathological conditions, ND-MWCNT exposure may play a role in the initiation of these diseases. [Description provided by NIOSH]
• Subjects:
  Animals Carcinogens Electricity Fibrosis Laboratories Lung Lung Diseases Occupational Health Respiratory System Respiratory Tract Diseases Safety Toxicology

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• Keywords:
  Carcinogenesis Cell-damage Cell-function Cellular-function Electrical-conductivity Exposure-levels In-vitro-study In-vivo-study Laboratory-animals Molecular-structure Nanotechnology Pulmonary-disorders Pulmonary-function Pulmonary-system Pulmonary-system-disorders

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  138
• Issue:
  1
• NIOSHTIC Number:
  nn:20043889
• Citation:
  Toxicologist 2014 Mar; 138(1):155
• Federal Fiscal Year:
  2014
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 53rd Annual Meeting and ToxExpo, March 23-27, 2014, Phonex, Arizona
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:2f461db3153f0dd43abefd8dc2735087eacd58d1e7b3e770dc1fbebd146169eb334b4fb7ea44ac95a80f7756e56fc61c30ecc08d87a44fd2710ed52c90185075
• Download URL:
  https://stacks.cdc.gov/view/cdc/199762/cdc_199762_DS1.pdf
• File Type:
  [PDF - 245.54 KB ]