Induction of Apoptosis from Instillation of Agricultural Dust

Details

• Personal Author:
  Antonini JM ; Castranova, Vincent ; Mercer RR ; Scalilloni JF ; Vallyathan V ; Wang L
• Description:
  In this study, we test the hypothesis that induction of alveolar macrophages (AM) apoptosis plays a role in lung injury from particulates. We examined AM apoptosis in F344 rats following intratracheal instillation of 1ml of saline containing 4mg of particles. Particles used include crystalline silica, titanium dioxide and particulates from air sampling of citrus and grape fields. After 4 weeks, rats were sacrificed and lung sections analyzed for the number of apoptotic cells per lung using TdT labeling. Apoptotic macrophages, expressed in millions of cells per lung (mean+SE,N=4) were 43.4+4 for crystalline silica, 10.50+0.2 for grape, 8.1+0.5 for citrus and 3.1+0.4 for titanium dioxide. Saline controls were 1.6+0.4. The degree of apoptosis correlated well with inflammation and damage suggesting a role for apoptosis in lung injury resulting from particulates. [Description provided by NIOSH]
• Subjects:
  Agriculture Aluminum Silicates Animals Dust Environmental Monitoring Laboratories Lung Lung Diseases Occupational Health Particulate Matter Respiratory System Respiratory Tract Diseases Safety Silicon Dioxide

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• Keywords:
  Agricultural-industry Air-samples Air-sampling Alveolar-cells Animal-studies Dust-exposure Dust-particles Dusts Laboratory-animals Lung-disorders Lung-function Lung-irritants Particulate-dust Particulates Pulmonary-system-disorders Respiratory-system-disorders Silica-dusts Silicates

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• ISSN:
  0892-6638
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings
• Place as Subject:
  Louisiana ; OSHA Region 3 ; OSHA Region 6 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  16
• Issue:
  5
• NIOSHTIC Number:
  nn:20023059
• Citation:
  FASEB J 2002 Mar; 16(5):A962
• Contact Point Address:
  NIOSH, 1095 Willowdale Road, Morgantown, WV 26505
• CAS Registry Number:
  Quartz (SiO2) (CAS RN 14808-60-7) ; Titania (CAS RN 13463-67-7)
• Federal Fiscal Year:
  2002
• Peer Reviewed:
  False
• Source Full Name:
  The FASEB Journal, Experimental Biology 2002, New Orleans, Louisiana, April 20-24, 2002
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:09421469eccd5ec9a707dec1f2527e1ee9fde4147c5599e3038952d888dbd8e18dfe60edf6e9372540f818eeb1305721bf7466177e83bf8c616f19dbbebdd0b0
• Download URL:
  https://stacks.cdc.gov/view/cdc/199643/cdc_199643_DS1.pdf
• File Type:
  [PDF - 525.65 KB ]