Silica exposure increases nitric oxide (NO) production in rat alveolar type II cell fractions

Details

• Personal Author:
  Castranova, Vincent ; Huffman LJ ; Judy JD ; Pailes WH
• Description:
  Alveolar type II epithelial (type II) cells play an important role in the pulmonary response to irritant challenges. Recent evidence suggests that these cells produce NO which may, in turn, participate in defense reactions in response to foreign substances. In the present study, we assessed whether the intratracheal (IT) instillation of silica might be associated with an increase in NO production by rat type II cells. Male rats received IT instillations of endotoxin-free saline or silica (10 mg/100 gBW). After 24 hrs, type II cell-enriched fractions were isolated from perfused/lavaged lungs by elastase/collagenase digestion and purified by centrifugal elutriation. Inducible NO synthase (iNOS) mRNA levels in freshly isolated type II cell fractions were evaluated using Northern blot analysis. Media nitrate and nitrite levels were indexed after a 20 hr incubation period of type II cells in culture. iNOS mRNA levels were elevated in the type IT cell fractions isolated from silica-treated rats compared to saline-controls. After culture, total media nitrate and nitrite levels were three-fold higher in silica vs saline-exposed type II cell fractions. These results suggest that NO synthase expression is induced in rat alveolar type II epithelial cells in response to an in vivo silica challenge. [Description provided by NIOSH]
• Subjects:
  Aluminum Silicates Animals Irritants Laboratories Lung Lung Diseases Occupational Health Respiratory System Respiratory Tract Diseases Safety Silicon Dioxide

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• Keywords:
  Alveolar-cells Animal-studies Laboratory-animals Lung-cells Lung-disorders Lung-irritants Pulmonary-system-disorders Respiratory-irritants Respiratory-system-disorders Silica-dusts Silicates

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• ISSN:
  0892-6638
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  8
• Issue:
  4
• NIOSHTIC Number:
  nn:20038100
• Citation:
  FASEB J 1994 Mar; 8(4):A147
• CAS Registry Number:
  Nitric oxide (CAS RN 10102-43-9) ; Quartz (SiO2) (CAS RN 14808-60-7)
• Federal Fiscal Year:
  1994
• Peer Reviewed:
  False
• Source Full Name:
  The FASEB Journal. Experimental Biology '94, Anaheim, California, April 24-28, 1994
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:49bf2eb31de881a4fa63cd81b7a9134c78867d1814353ef5d57a2ababc8220a728fd7029bf902caaf456790e66abfc8b3ae160b4b4d1a3139962acd0a7452238
• Download URL:
  https://stacks.cdc.gov/view/cdc/191916/cdc_191916_DS1.pdf
• File Type:
  [PDF - 404.23 KB ]