Effects of Paving Asphalt Fume Exposure on Genotoxic and Mutagenic Activities in the Lung

Details

• Personal Author:
  Barger M ; Castranova, Vincent ; Frazer D ; Ma J ; Stone S ; Tomblyn S ; Yin X ; Zhao H
• Description:
  Asphalt fumes are complex mixtures of aerosols and vapors containing various organic compounds, including polycyclic aromatic hydrocarbons, and have been demonstrated to alter the cytochrome P-450 system in the lung with significant induction of CYPIAl, The present study was carried out to characterize the potential mutagenic and genotoxic effects of inhaled asphalt fume in a rat model, Rats were exposed to air or asphalt fume generated at paving temperature (-150 degreesC) at --98 mgim3 and 6h/day for 5 days Alveolar macrophages (AM) were obtained by bronchoalveolar lavage, S9 was isolated from the lung tissue, Mutagenicity was monitored using the Ames test and genotoxicity was determined by Comet assay. In comparison to controls, AM from asphalt fume-exposed rats showed significant DNA damage as demonstrated by the Comet assay. However, the 89 fraction from asphalt exposed rat lungs used for metabolic activation of 2-aminoanthracene or benzo[a]pyrene in Ames tests, did not induce mutagenic activity in either S. typhimurium YGIO24 or YGIO29. These results suggest that short term inhalation of asphalt fume did not induce 89 metabolic activity-dependent mutagenicity, but caused significant DNA damage in AM. [Description provided by NIOSH]
• Subjects:
  Aerosols Air Pollutants, Occupational Animals Digestive System Energy Metabolism Hydrocarbons Laboratories Liver Lung Mutagens Occupational Health Polycyclic Aromatic Hydrocarbons Respiratory System Safety Solvents

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• Keywords:
  Aerosol-particles Animal-studies Asphalt-fumes Exposure-levels Fumes Genotoxic-effects In-vitro-study Laboratory-animals Liver-enzymes Lung-cells Lung-tissue Metabolic-activation Mutagenicity Organic-compounds Polycyclic-aromatic-hydrocarbons Vapors

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• ISSN:
  0892-6638
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings
• Place as Subject:
  Louisiana ; OSHA Region 3 ; OSHA Region 6 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  16
• Issue:
  5
• NIOSHTIC Number:
  nn:20023052
• Citation:
  FASEB J 2002 Mar; 16(5):A962
• Contact Point Address:
  NIOSH, 1095 Willowdale Road, Morgantown, WV 26505
• CAS Registry Number:
  Benzo[a]pyrene (CAS RN 50-32-8)
• Federal Fiscal Year:
  2002
• NORA Priority Area:
  Work Environment and Workforce: Mixed Exposures
• Peer Reviewed:
  False
• Source Full Name:
  The FASEB Journal, Experimental Biology 2002, New Orleans, Louisiana, April 20-24, 2002
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:3caccc957ec060ba3283c57845ba267a1aa66b461ce305b26a5b7a1b830e57d752a190d5fcb598777407c87c1b6229731e4c850dcd015f5b1b37f0f6c1957565
• Download URL:
  https://stacks.cdc.gov/view/cdc/199637/cdc_199637_DS1.pdf
• File Type:
  [PDF - 110.86 KB ]