Bioeletric Response of Guinea-Pig Tracheal Epithelium Hypotonic Solution

Details

• Personal Author:
  Fedan, Jeffery S. ; Knudsen GA ; Leonette JW ; Van Scott MR
• Description:
  Hypotonic solution causes cell swelling. Hypotonic solution in the lumen of the GP trachea depolarizes the E and contracts the airway smooth muscle. This study examined the effects of mucosal hypotonic solution on the bioelectric properties of GP tracheal E mounted in Ussing chambers. Stepwise dilution of the mucosal modified Krebs-Henseleit (MKH) solution (1 % to 50% with water) progressively decreased transepithelial short circuit current (I,,) and increased resistance (R,). Responses to hypo tonicity were unaffected by 1 11M MK-571, 5 11M piriprost, or 10 U/ml apyrase added bilaterally, indicating that L TD4 and ATP were not involved. Mucosal amiloride (30 1iM) decreased basal Isc (10%) but did not affect responses to hypotonicity. Mucosal NPPB (100 11M) decreased basal Isc (88%). Hypotonicity in the presence of NPPB reversed the polarity of Isc and inhibited the increase in R,. but the (delta) Isc was unaffected. Isc also was decreased after reducing [NaCl] in the mucosal bath (113-85 nM). These results indicate that basal across GP tracheal E is mainly due to Cl secretion. The (delta) Isc induced by hypotonicity is consistent with passive transepithelial flux of Na (S-M). [Description provided by NIOSH]
• Subjects:
  Laboratories Occupational Health Safety
• Keywords:
  Bioelectric-effects Cell-damage Cell-function Laboratory-animals
• ISSN:
  0892-6638
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  16
• Issue:
  5
• NIOSHTIC Number:
  nn:20023043
• Citation:
  FASEB J 2002 Apr; 16(5):A57
• Federal Fiscal Year:
  2002
• Peer Reviewed:
  False
• Source Full Name:
  The FASEB Journal, Experimental Biology 2002, New Orleans, Louisiana, April 20-24, 2002
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:eb9ddac1bccf53163e89d3a05c87fee25e153a1f28311df1aaaf3adc1fd97a05c9a184eefae00c28c337b40739892d3738d1d998e4b45dcfeacd833f9d0dce02
• Download URL:
  https://stacks.cdc.gov/view/cdc/199629/cdc_199629_DS1.pdf
• File Type:
  [PDF - 135.58 KB ]