Time-Course of Functional and Pathological Changes in Chlorine Exposed A/J Mice

Details

• Personal Author:
  Campbell HR ; Martin JG ; Ramos-Barbon D ; Tuck SA
• Description:
  Inhalation of irritants such as chlorine (CI,) can lead to the development of irritant induced asthma. Few animal models have been developed to study the effects of CI, on airway) cellular responses and changes in lung function. To investigate in vivo the effects of CI2 on airway inflammation and lung function, NJ mice were exposed to inhaled CI, (400 ppm) for 5 min. Animals were assessed 24h, 48h and 7 d after exposure by measuring respiratory system resistance (RRS) and elastance (ELRS) in response to i.v. methacholine (MCh). BAL was performed to determine total and differential cell counts and epithelial cell shedding. To determine the effects of inhibiting iNOS, a group of animals was treated with I400W in addition to being exposed to Cl, (400 ppm) for 5 minutes and studied 24 h after 0, exposure. Maximal inflammatory response to Cl, was observed 24 h after CI, expos... and was characterized by increased BAL total cell counts (n=6; p<0.001), granulocytes (n=6;p<0.002), macrophages (n=6;p<0.001) and epithelial cells (n=6;p
• Subjects:
  Animals Chlorine Compounds Inhalation Exposure Laboratories Lung Occupational Health Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Animal-studies Cellular-function Chlorine-compounds In-vivo-study Inhalants Laboratory-animals Lung-function Pulmonary-system-disorders Respiratory-system-disorders
• ISSN:
  1073-449X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Abstract or Summary ; Proceedings
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  165
• Issue:
  8
• NIOSHTIC Number:
  nn:20023021
• Citation:
  Am J Respir Crit Care Med 2002 Apr; 165(8)(Suppl):A522
• CAS Registry Number:
  Chlorine (CAS RN 7782-50-5)
• Federal Fiscal Year:
  2002
• NORA Priority Area:
  Disease and Injury: Asthma and Chronic Obstructive Pulmonary Disease
• Performing Organization:
  Hospital du Sacre-Coeur, Montreal, Quebec
• Peer Reviewed:
  False
• Start Date:
  19990930
• Source Full Name:
  American Journal of Respiratory and Critical Care Medicine, 2002 International Conference, The American Thoracic Society, Atlanta Georgia, May 17-22, 2002
• Supplement:
  Suppl
• End Date:
  20021229
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:e3b0592c452b2dc715e413161454a55aadab50bded9b7edb8c3a81495e2358188af7fca236f76e63136a4b242dc95c339c052d6b9104f70babc6b77077b5afb5
• Download URL:
  https://stacks.cdc.gov/view/cdc/199611/cdc_199611_DS1.pdf
• File Type:
  [PDF - 189.34 KB ]