Toward Mechanism-Based Antioxidant Interventions: Lessons from Natural Antioxidants

Details

• Personal Author:
  Kagan VE ; Kawai K ; Kisin ER ; Osipov AN ; Serbinova EA ; Serinkan BF ; Shvedova AA ; Wolinsky I
• Description:
  It is generally accepted that one of the major and important contributions to skin aging, skin disorders, and skin diseases results from reactive oxygen species. More than other tissues, the skin is exposed to numerous environmental chemical and physical agents, such as ultraviolet light, causing oxidative stress. Accelerated cutaneous UV-induced aging, photo aging, is only one of the harmful effects of continual oxygen radical production in the skin. Interestingly, our ELISA assays of 8-oxo-2'-deoxyguanosine in skin of young and old Balb/c mice showed that cumene hydroperoxide-induced accumulation of the biomarker of oxidative DNA damage in skin of 32-week-old mice occurred independently of their vitamin E status, while no accumulation of oxo8-dG was detectable in the skin of young animals. This suggests that vitamin E is not the major protector of skin against cumene hydroperoxide-induced oxidative stress. Production and accumulation of apoptotic cells is one of the characteristic features of skin damage by oxidative stress that, in the absence of effective scavenging by macrophages, dramatically enhances oxidative damage and inflammatory response. In our model experiments, we demonstrated that Cu-OOH induces significant oxidative stress in phospholipids of normal human epidermal keratinocytes (NHEK) whose characteristic feature is an early and profound oxidation of phosphatidylserine (PS), likely related to PS externalization. Since externalized PS is a signal for recognition of apoptotic cells by macrophage scavenger receptors, PS oxidation may be translatable into elimination of thus damaged NHEKs. Experiments are now underway to determine whether inhibition of PS oxidation by antioxidants may interfere with important signaling functions of oxidative stress in eliminating apoptotic cells. [Description provided by NIOSH]
• Subjects:
  Age Factors Animals Laboratories Occupational Health Radiation Safety Skin Skin Diseases Ultraviolet Rays
• Keywords:
  Age-factors Animal-studies Author Keywords: Vitamin E Laboratory-animals Natural Antioxidants Oxidation Prooxidant Signaling Pathways Skin-diseases Skin-disorders Skin-exposure Ultraviolet-light Ultraviolet-radiation Vitamins

  More +
• ISBN:
  9780897665247
• ISSN:
  0077-8923
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 3 ; OSHA Region 6 ; OSHA Region 9 ; Pennsylvania ; Texas ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  188-198
• Volume:
  959
• Issue:
  1
• NIOSHTIC Number:
  nn:20022873
• Citation:
  Ann NY Acad Sci 2002 Apr; 959(1):188-198
• Contact Point Address:
  Valerian E. Kagan, 3343 Forbes Ave., Pittsburgh, PA 15260. Voice 412-383-2136
• Email:
  kagan@pitt.edu
• Federal Fiscal Year:
  2002
• NORA Priority Area:
  Disease and Injury: Allergic and Irritant Dermatitis
• Peer Reviewed:
  True
• Source Full Name:
  Annals of the New York Academy of Sciences
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:2cc585bf14e71598b517516c6b6c52898b01b596a5cad2e8bdaf3ce72e9d60673fde16b6053a2a365c4ea9da938d8af1df52cc819126bdc3a721eb3902f22a3c
• Download URL:
  https://stacks.cdc.gov/view/cdc/199501/cdc_199501_DS1.pdf
• File Type:
  [PDF - 5.27 MB ]