Particulate 1–>3-Beta-Glucan Is the More Potent Form for Inducing Pulmonary Inflammation in Rats

Details

• Personal Author:
  Barger M ; Castranova, Vincent ; Frazer DG ; Robinson VA ; Young S
• Description:
  1-->3-Beta-Glucans, derived from the inner cell wall of yeasts and fungi, are commonly found in indoor air dust samples and have been implicated in organic dust toxic syndrome. In a previous study, we reported that 1-->3-Beta-glucan (zymosan A) induced acute pulmonary inflammation in rats. The present study investigates which form of 1-->3-Beta-glucans, particulate or soluble, is more potent in inducing pulmonary inflammation. Zymosan A was suspended with 0.25 N NaOH for 30 min, neutralized, dialyzed for 2 days against deionized water, and particulate and soluble fractions collected. Male Sprague-Dawley rats were exposed via intratracheal instillation to NaOH-soluble or NaOH-insoluble zymosan A (1.8 mg/ml, 0.26 ml). At 18 hrs post-exposure, various indicators of pulmonary response were monitored, including indicators of lung damage, such as serum albumin concentration and lactate dehydrogenase activity in acellular bronchoalveolar lavage fluid. Inflammation was characterized by an increase in lavageable polymorphonuclear leukocytes (PMN), pulmonary irritation (breathing frequency increase) and oxidant production (nitric oxide and chemiluminescence (CL)). Exposure to the particulate fraction of NaOH-treated zymosan caused a significant increase in all these indicators. In contrast, rats exposed to the NaOH-soluble fraction did not show significant increase for most of these indicators except for albumin, PMN and CL. However, these increases were significantly smaller than with exposure to NaOH-insoluble zymosan. Therefore, the results demonstrate that particulate zymosan A is more potent in inducing pulmonary inflammation and damage in rats than the soluble form of this Beta-glucan. [Description provided by NIOSH]
• Subjects:
  Aerosols Air Pollution, Indoor Animals Dust Environmental Health Fungi Laboratories Lung Lung Diseases Occupational Health Respiratory System Respiratory Tract Diseases Safety

  More +
• Keywords:
  Animal-studies Indoor-air-pollution Indoor-environmental-quality Laboratory-animals Organic-dusts Pulmonary-disorders Pulmonary-system-disorders Respiratory-system-disorders Yeasts
• ISSN:
  1096-6080
• Publisher:
  Society of Toxicology
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  66
• NIOSHTIC Number:
  nn:20022823
• Citation:
  Toxicologist 2002 Mar; 66(1-S):354
• Federal Fiscal Year:
  2002
• NORA Priority Area:
  Work Environment and Workforce: Mixed Exposures
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 41st Annual Meeting and ToxExpo, March 17-21, 2002, Nashville, Tennessee
• Supplement:
  1-S
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:5e1c47894e961e7f521f2aa784b00dd24578dc1953c18a5a0d2c795a1a88397a39d2e4597315ff3a554d15aa94a9be09a16d6cfb86fab0a60432670bd954bc1d
• Download URL:
  https://stacks.cdc.gov/view/cdc/199460/cdc_199460_DS1.pdf
• File Type:
  [PDF - 181.86 KB ]