Ozone-Enhanced Airway Hyperrespomsiveness Involves Intrinsic Airway Neurons in Ferret Trachea

Details

• Personal Author:
  Dey RD ; Fedan, Jeffery S. ; Satterfield BE ; Wu ZX
• Description:
  Exposure to ozone (O3), a major air pollutant in urban areas, induces airway hyperresponsiveness mediated partly by the release of tachykinins from nerve terminals of intrinsic airway ganglia (Wu et. al., J. Appl. Physiol. 91:371-378, 2001). The purpose of this study was to investigate the possible involvement of intrinsic airway neurons in O3-induced airway hyperresponsiveness. Segments of ferret trachea were exposed in vitro to 2 ppm 03 or air for 1 hr. Reactivity of isolated tracheal smooth muscle strips to methacholine was significantly increased after 03-exposure, as were contractions to electrical field stimulation (EFS). The 03-enhanced responsiveness was maintained in tracheal segments cultured for 24 h, a procedure shown to deplete most sensory nerves while maintaining viability of intrinsic airway neurons. Furthermore, segments of trachea cultured with 3x10-6 M capsaicin for 24h, which completely depletes tachykinins in sensory nerves, did not abolish the 03-enhanced reactivity to cholinergic agonists and EFS. The findings support the hypotheses that 03-induced airway smooth muscle hyperresponsiveness results partly from the activation intrinsic airway neurons. Further, these neurons may be activated by reflexes ot mediators originating in the airway [Description provided by NIOSH]
• Subjects:
  Methacholine Compounds Occupational Health Respiratory System Safety
• Keywords:
  Airway-obstruction Airway-resistance Cholinergic-receptors Electrical-stimulation In-vitro-study Methacholines Neuropathy Reflexes
• ISSN:
  1073-449X
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  165
• Issue:
  8
• NIOSHTIC Number:
  nn:20022803
• Citation:
  Am J Respir Crit Care Med 2002 Apr; 165(8)(Suppl):A718
• CAS Registry Number:
  Ozone (CAS RN 10028-15-6)
• Federal Fiscal Year:
  2002
• NORA Priority Area:
  Research Tools and Approaches: Exposure Assessment Methods
• Peer Reviewed:
  False
• Source Full Name:
  American Journal of Respiratory and Critical Care Medicine, 2002 International Conference, The American Thoracic Society, Atlanta Georgia, May 17-22, 2002
• Supplement:
  Suppl
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:8c1cf306b3535aa5bc57806eceefda9de4459af0e86a437a813b6117443b8896237d4283b88ee814e9a4ebf8f5f29216d8a2445550b32b0c76f3e2337aa02e23
• Download URL:
  https://stacks.cdc.gov/view/cdc/199444/cdc_199444_DS1.pdf
• File Type:
  [PDF - 323.77 KB ]