A Novel Technique to Study Percutaneous Absorption by Using a Silastic Membrane Coated Fiber

Details

• Personal Author:
  Baynes RE ; Leidy RB ; Monteiro-Riviere NA ; Riviere JE ; Xia XR
• Description:
  Silastic membrane coated on a section of fused silica fiber has been used successfully as an analytical tool to extract analytes from aqueous solution. In this study, the silastic membrane coated fiber was used as a permeation membrane to study the percutaneous absorption of model pesticides. It represents a half compartment of the conventional Franz diffusion cell, which allows for more detailed permeation kinetics to be investigated. The membrane coated fiber was immersed in the magnetic stirred donor phase to adsorb the permeants from the solution, the fiber was then transferred directly into the injection port of a Gas Chromatograph/Mass Spectrometer for quantitative analysis. This technique can assess the permeation amounts at various permeation times, stirring speeds and donor compositions. A theoretical model was proposed to describe the permeation processes of the silastic membrane coated fiber in terms of diffusion parameters. This model allows for the boundary layer between the donor phase and the silastic membrane to be considered, it's thickness is assumed to be constant under steady-state diffusion. The permeation kinetics of 30 different compounds having a wide range of partition coefficients were examined utilizing a silastic membrane coated fiber. The experimental permeation flux and time profiles were well described by the proposed mathematical model. The thickness of the boundary layer, the diffusion coefficients in the donor phase and in the silastic membrane, and the partition coefficients can be measured simultaneously with this novel technique. This approach would foster development of high-throughput determination of skin diffusion parameters. [Description provided by NIOSH]
• Subjects:
  Magnetic Fields Mass Spectrometry Occupational Health Particulate Matter Safety Skin Skin Absorption
• Keywords:
  Diffusion-analysis Fiber-deposition Gas-chromatography Magnetic-fields Mass-spectrometry Skin-absorption
• ISSN:
  1096-6080
• Publisher:
  Society of Toxicology
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Abstract or Summary
• Place as Subject:
  North Carolina ; OSHA Region 4 ; Tennessee
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  66
• NIOSHTIC Number:
  nn:20022788
• Citation:
  Toxicologist 2002 Mar; 66(1-S):164
• Federal Fiscal Year:
  2002
• NORA Priority Area:
  Disease and Injury: Allergic and Irritant Dermatitis ; Work Environment and Workforce: Mixed Exposures
• Performing Organization:
  North Carolina State University, Raleigh, North Carolina
• Peer Reviewed:
  False
• Start Date:
  20000801
• Source Full Name:
  The Toxicologist. Society of Toxicology 41st Annual Meeting and ToxExpo, March 17-21, 2002, Nashville, Tennessee
• Supplement:
  1-S
• End Date:
  20150731
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:04b8b9bf04e98a2a054b40aa5e34fa77ccf1adb32280f3b094e18c5edc726432f7f45183748cd2dc4beca2d64c571870ad8aa2b326cea03b402ed028fb71e453
• Download URL:
  https://stacks.cdc.gov/view/cdc/199434/cdc_199434_DS1.pdf
• File Type:
  [PDF - 163.11 KB ]