Effects of Phospholipid Surfactant on Apoptosis Induction by Respirable Quartz and Kaolin in NR8383 Rat Pulmonary Macrophages

Details

• Personal Author:
  Gao N ; Keane MJ ; Miller, William E. ; Ong T ; Wallace WE ; Ye J
• Description:
  Apoptosis was measured in rat alveolar macrophage NR8383 cells challenged in vitro with respirable quartz or kaolin dust and with the dusts pretreated with dipalmitoyl phosphatidylcholine (DPPC) to model conditioning of respired dusts by interaction with a primary phospholipid component of pulmonary surfactant. Quartz dust is known to induce apoptosis in vitro and in vivo. For this study, quartz and kaolin were compared as dusts of similar cytotoxicity in some in vitro assays but of differing pathogenic potential: quartz can cause significant pulmonary fibrosis while kaolin generally does not. NR8383 cells exposed to native quartz at concentrations from 50 to 400 microg/ml for 6 h showed a dose-dependent increase in apoptosis measured by the TdT-mediated dUTP-fluorescein nick end labeling (TUNEL), cell death ELISA, and DNA ladder formation assays, while native kaolin induced significant response only at the higher concentrations and only in the TUNEL and ELISA assays. For cell challenge from 6 h to 5 days at 100 microg/ml of dust, quartz was active at all times while kaolin was active only at 5 days. DPPC pre-treatment suppressed quartz activity until 3 days and kaolin activity through 5 days. Cellular release of lactate dehydrogenase, measured in parallel experiments to compare dust apoptotic and necrotic activities, indicated that components of serum as well as surfactant may affect kaolin in vitro expression of those activities. [Description provided by NIOSH]
• Subjects:
  Cytotoxins DNA Dust Fibrosis Lung Lung Diseases Neoplasms Occupational Health Quartz Respiratory System Respiratory Tract Diseases Safety Silicon Dioxide

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• Keywords:
  Author Keywords: Apoptosis Cell Death ELISA Assay Cytotoxicity Dipalmitoylphosphatidylcholine DNA-damage DNA Ladder Formation Assay Dose-response Drug-interaction Kaolin Lactate Dehydrogenase Assay Lung-cancer Lung-fibrosis Pneumoconiosis Pulmonary-system-disorders Pulmonary Fibrosis Pulmonary Surfactant Quartz Quartz-dust Rat Macrophage NR8383 Respirable Dust Respiratory-system-disorders Serum Silica-dusts Surfactant TUNEL Assay

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• ISSN:
  0041-008X
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  217-225
• Volume:
  175
• Issue:
  3
• NIOSHTIC Number:
  nn:20022489
• Citation:
  Toxicol Appl Pharmacol 2001 Sep; 175(3):217-225
• Contact Point Address:
  National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, West Virginia, 26505
• CAS Registry Number:
  1,2-Dipalmitoylphosphatidylcholine (CAS RN 2644-64-6) ; Kaolin (CAS RN 1332-58-7) ; Quartz (SiO2) (CAS RN 14808-60-7)
• Federal Fiscal Year:
  2001
• Peer Reviewed:
  True
• Source Full Name:
  Toxicology and Applied Pharmacology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:ce01df6f525a0e1f33f5fbf2e03ca6c626a240c879d755731ac27f6f0f92df25d50d972054daf636867eaec22a40514bb6f5b3b47f369087d73d0b6531b2aaf8
• Download URL:
  https://stacks.cdc.gov/view/cdc/199249/cdc_199249_DS1.pdf
• File Type:
  [PDF - 338.69 KB ]