Deficiency in Plasma Membrane Calcium ATPase Isoform 2 Increases Susceptibility to Noise-Induced Hearing Loss in Mice

Details

• Personal Author:
  Davis R ; Erway L ; Kozel P ; Krieg, Edward Jr. ; Shull G
• Description:
  Susceptibility to noise-induced hearing loss (NIHL) is poorly understood at the genetic level. Mice homozygous for a null mutation in the plasma membrane Ca2+-ATPase isoform 2 (PMCA2) gene are deaf (Kozel et al., 1998). PMCA2 is expressed on outer hair cell stereocilia (Furuta et al., 1998). Fridberger et al. (1998) observed that the outer hair cell cytoplasmic Ca2+ concentration rises following acoustic overstimulation. We hypothesized that Pmca2+/- mice may be more susceptible to NIHL. Since the auditory brainstem response (ABR) thresholds of Pmca2+/- mice vary with the presence of a modifier locus (Noben-Trauth et al., 1997), Pmca2+/- mice were outcrossed to normal hearing CAST/Ei mice. The pre-exposure ABR thresholds of the resulting Pmca2+/+ and Pmca2+/- siblings were indistinguishable. Groups of these mice were exposed to varying intensities of broadband noise, and ABR threshold shifts were calculated. Fifteen days following an 8 h, 113 dB noise exposure, the Pmca2+/- mice displayed significant (P < or = 0.0007) permanent threshold shifts at 16 and 32 kHz that were 15 or 25 dB greater than those observed in Pmca2+/+ littermates. Pmca2 may be the first gene with a known mutated protein product that confers increased susceptibility to NIHL. [Description provided by NIOSH]
• Subjects:
  Animals Genetics Hearing Loss, Noise-Induced Occupational Health Safety
• Keywords:
  Auditory-system Author Keywords: Mice Calcium Enzymology Genetic-factors Humans Noise-induced-hearing-loss Noise-induced Hearing Loss Plasma Membrane Calcium ATPase Isoform 2
• ISSN:
  0378-5955
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  231-239
• Volume:
  164
• NIOSHTIC Number:
  nn:20022399
• Citation:
  Hear Res 2002 Feb; 164(1-2):231-239
• Contact Point Address:
  Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati, OH 45267, USA
• Email:
  rrd1@cdc.gov
• Federal Fiscal Year:
  2002
• Peer Reviewed:
  True
• Source Full Name:
  Hearing Research
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:9b5dc46bf5910474700dec7a5486fbd17ca04a9a8c94adcc2a63f216df1b32003df22199bb64d621b599269e3b25e77b14f664c8f1eb58e7a3f325ca1717a723
• Download URL:
  https://stacks.cdc.gov/view/cdc/199191/cdc_199191_DS1.pdf
• File Type:
  [PDF - 433.52 KB ]