p38 Signaling-Mediated Hypoxia-Inducible Factor 1 Alpha and Vascular Endothelial Growth Factor Induction by Cr(VI) in DU145 Human Prostate Carcinoma Cells

Details

• Personal Author:
  Antonini J ; Castranova, Vincent ; Corum L ; Flynn DC ; Gao N ; Jiang BH ; Leonard, Stephen S. ; Roberts, Jennifer R. ; Shi X ; Zhang Z ; Zheng JZ
• Description:
  Chromium(VI) (Cr(VI)) is widely used in industry and is a potent inducer of tumors in animals. The present study demonstrates that Cr(VI) induces hypoxia-inducible factor 1 (HIF-1) activity through the specific expression of HIF-1alpha but not HIF-1beta subunit and increases the level of vascular endothelial growth factor (VEGF) expression in DU145 human prostate carcinoma cells. To dissect the signaling pathways involved in Cr(VI)-induced HIF-1 expression, we found that p38 mitogen-activated protein kinase signaling was required for HIF-1alpha expression induced by Cr(VI). Neither phosphatidylinositol 3-kinase nor extracellular signal-regulated kinase activity was required for Cr(VI)-induced HIF-1 expression. Cr(VI) induced expression of HIF-1 and VEGF through the production of reactive oxygen species in DU145 cells. The major species of reactive oxygen species responsible for the induction of HIF-1 and VEGF expression is H(2)O(2). These results suggest that the expression of HIF-1 and VEGF induced by Cr(VI) may be an important signaling pathway in the Cr(VI)-induced carcinogenesis. [Description provided by NIOSH]
• Subjects:
  Biochemistry Carcinogens Chemistry Chromium Genetics Lung Lung Diseases Neoplasms Occupational Health Respiratory System Respiratory Tract Diseases Safety Urogenital Diseases Urogenital System

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• Keywords:
  Biochemical-analysis Biochemical-tests Cancer Carcinomas Cell-biology Cell-growth Cellular-function Chemical-kinetics Chemical-properties Chemical-reactions Chromium-compounds Gene-mutation Genes Genotoxic-effects Humans Hypoxia Immunological-tests Mutation Prostate-cancer Tumors

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• ISSN:
  0021-9258
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  277
• Issue:
  47
• NIOSHTIC Number:
  nn:20022382
• Citation:
  J Biol Chem 2002 Nov; 277(47):45041-45048
• Contact Point Address:
  Mary Babb Randolph Cancer Center, Department of Microbiology, Immunology and Cell Biology, West Virginia University, Morgantown, West Virginia 26506-9300, USA
• Email:
  bhjiang@hsc.wvu.edu
• CAS Registry Number:
  Chromic trioxide (CAS RN 1333-82-0)
• Federal Fiscal Year:
  2003
• NORA Priority Area:
  Research Tools and Approaches: Cancer Research Methods
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Biological Chemistry
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:149c646d817c292811311abb88f1ebc8b0b1a77303964272779095cc4c306d6b88f9658a3babccbfa2b3578151942675424291e8154fc52ee9a68d7b68d683b0
• Download URL:
  https://stacks.cdc.gov/view/cdc/199179/cdc_199179_DS1.pdf
• File Type:
  [PDF - 439.45 KB ]