Gene Structure, Tissue Expression, and Linkage Mapping of the Mouse DLC-1 Gene (Arhgap7)

Details

• Personal Author:
  Durkin ME ; Popescu NC ; Thorgeirsson SS ; Yuan BZ
• Description:
  DLC-1 (deleted in liver cancer 1) is a candidate tumor suppressor gene for hepatocellular carcinoma and other cancers. It is the human homologue of rat p122, which has been shown to function as a GTPase activating protein for RhoA, and it may be involved in signal transduction pathways regulating cell proliferation and adhesion. To establish an animal model for studying the regulation and function of DLC-1, we have undertaken the characterization of the mouse DLC-1 gene. Northern blot analysis shows that the mouse DLC-1 mRNA is widely expressed, with the highest levels in heart, liver, and lung. Mouse genomic clones that contain the entire DLC-1 gene of 47 kb were isolated. The mouse gene consists of 14 exons, and the structural organization is highly similar to that of the human gene. The promoter region of the mouse gene was GC-rich and contained potential binding sites for transcription factors SP1, GCF, and AP-2. A polymorphic microsatellite marker in intron 8 was used for mapping the gene (Arhgap7) to 20 cM on mouse chromosome 8 and for allelotyping of mouse liver tumor DNAs. [Description provided by NIOSH]
• Subjects:
  Amino Acids Animals Carcinogens Digestive System Digestive System Diseases DNA Genetics Liver Neoplasms Occupational Health Safety

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• Keywords:
  Amino-acids Animal-studies Author Keywords: Chromosome 8 Cancer Carcinomas DNA-damage Gene-mutation Genes Genetic-disorders Genetic-factors Genotoxic-effects Liver-cancer Liver-cells Liver-tumors Oncogenic-agents Promoter Proteins Rho GAP Tumor-inhibition Tumorigenesis Tumors Tumor Suppressor Gene

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• ISSN:
  0378-1119
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  119-127
• Volume:
  288
• Issue:
  1
• NIOSHTIC Number:
  nn:20022335
• Citation:
  Gene 2002 Apr; 288(1-2):119-127
• Contact Point Address:
  Laboratory of Experimental Carcinogenesis, Building 37, Room 3C28, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
• Email:
  marian_durkin@nih.gov
• Federal Fiscal Year:
  2002
• Peer Reviewed:
  True
• Source Full Name:
  Gene
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:a8e0656dda997765960ed27a5cde440eed6b62e51d4ea1b3d818d48e35cd9561f368754937a49be2bfd3642d2be6cdc8f16a197dfbbb69d77b58fe0aaec07d4d
• Download URL:
  https://stacks.cdc.gov/view/cdc/199145/cdc_199145_DS1.pdf
• File Type:
  [PDF - 287.90 KB ]