Chemoprotection by Phenolic Antioxidants

Details

• Personal Author:
  Kinneer K ; Ma Q
• Description:
  Phenolic antioxidants exhibit anti-inflammatory activity in protection against chemical toxicity and cancer. To investigate the molecular mechanism of anti-inflammation, we analyzed the regulation of tumor necrosis factor (TNF-) expression in macrophages, a key step in inflammation, by the antioxidants. Whereas lipopolysaccharide (LPS), an inflammatory inducer, stimulates rapid synthesis of TNF- protein, phenolic antioxidants, exemplified by tert-butyl hydroquinone and 1,4-dihydroquinone, block LPS-induced production of TNF- protein in a time- and dose-dependent manner. Inhibition of TNF- induction correlates with the capacity of the antioxidants to undergo oxidation-reduction cycling, implicating oxidative signaling in the inhibition. The antioxidants blocked LPS-induced increase of the steady-state mRNA of TNF- but did not affect the half-life of the mRNA. Electrophoretic mobility shift assay reveals a total inhibition of LPS-induced formation of nuclear factor B·DNA binding complexes by phenolic antioxidants. Finally, 1,4-dihydroquinone blocks the induction of TNF- target genes interleukin 1 and interleukin 6 at both mRNA and protein levels. Our findings demonstrate that phenolic antioxidants potently inhibit signal-induced TNF- transcription and suggest a mechanism of anti-inflammation by the antioxidants through control of cytokine induction during inflammation. [Description provided by NIOSH]
• Subjects:
  Antioxidants Neoplasms Occupational Health Phenol Safety
• Keywords:
  Cancer Humans Oxidation-reduction-reactions Phenols
• ISSN:
  0021-9258
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  277
• Issue:
  4
• NIOSHTIC Number:
  nn:20022294
• Citation:
  J Biol Chem 2002 Jan; 25(4):2477-2484
• Contact Point Address:
  CDC/NIOSH, Mail Stop 3014, 1095 Willowdale Rd., Morgantown, WV 26505
• Email:
  qam1@cdc.gov
• CAS Registry Number:
  Hydrogen peroxide (CAS RN 7722-84-1) ; Hydroquinone (CAS RN 123-31-9) ; Phenol (CAS RN 108-95-2) ; tert-Butylhydroquinone (CAS RN 1948-33-0)
• Federal Fiscal Year:
  2002
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Biological Chemistry
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:950d6aaacf6ec018eafae0a94bae3e2ba079994471ec9301632e59cbcae5dc777c7c6e5f1ab74cb97514a634ce916d8f129dc7b57f79fa786c1a4ef21a17b516
• Download URL:
  https://stacks.cdc.gov/view/cdc/199113/cdc_199113_DS1.pdf
• File Type:
  [PDF - 360.61 KB ]