The Effect of Diesel Exhaust Particles (DEP) and Carbon Black (CB) on Thiol Changes in Pulmonary Ovalbumin Allergic Sensitized Brown Norway Rats

Details

• Personal Author:
  Al-Humadi NH ; Barger MW ; Lewis, Daniel M. ; Ma JKH ; Ma JYC ; Siegel, Paul D. ; Weissman, David N.
• Description:
  Brown Norway rats were exposed by intratracheal instillation of saline, carbon black (CB), or diesel exhaust particles (DEP) (5 mg/kg) on day 1, followed by exposure to ovalbumin (OVA, 90 mg/m(3)) or saline for 30 minutes on days 1, 8, 15, and 29. Animals were sacrificed on day 30. The DEP, CB, or OVA exposure alone did not result in abnormal levels of inflammatory cells, lactate dehydrogenase (LDH), or total protein in the lavage fluid. In combined OVA-DEP or OVA-CB exposure, however, these markers were significantly increased. The adjuvant effect of CB and DEP on OVA sensitization was evidenced by the marked increases in serum OVA-specific IgG (5.6-fold) and IgE (3.5-4 fold) levels, and the increase in interleukin-4 (IL-4) mRNA levels in lung tissue. The OVA exposure markedly reduced glutathione (GSH) levels in both cell types. In combined DEP-OVA exposure, the level of GSH in lymphocytes was further decreased, indicating a possible interactive effect between DEP and OVA exposures. These results show that both DEP and CB augmented OVA-induced allergic sensitization, and that particle composition of DEP may not be a critical factor for the adjuvant effect. OVA exposure causes significant depletion of intracellular GSH in lymphocytes, which may play a key role in OVA-mediated immune responses. [Description provided by NIOSH]
• Subjects:
  Laboratories Lung Lung Diseases Occupational Health Particulate Matter Respiratory System Respiratory Tract Diseases Safety Vehicle Emissions
• Keywords:
  Author Keywords: Diesel Exhaust Particles Diesel-emissions Diesel-exhausts Immunoglobulin Interleukin-4 Laboratory-animals Lung-disorders Lung-irritants Ovalbumin Particulates Pulmonary-system-disorders Respiratory-system-disorders Thiols

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• ISSN:
  0190-2148
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  333-349
• Volume:
  28
• Issue:
  5
• NIOSHTIC Number:
  nn:20022189
• Citation:
  Exp Lung Res 2002 Jul-Aug; 28(5):333-349
• Contact Point Address:
  HELD, National Institute for Occupational Safety and Health, Morgantown, West Virginia
• Email:
  jma@wvu.edu
• CAS Registry Number:
  Carbon black (CAS RN 1333-86-4)
• Federal Fiscal Year:
  2002
• NORA Priority Area:
  Work Environment and Workforce: Mixed Exposures
• Peer Reviewed:
  True
• Source Full Name:
  Experimental Lung Research
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:10107e1e372376975b18e08c2d7035e72a94f3cb4b960efa7d93050845bb761db47cd9ca9e4fff5545ec04397a97b84c63dc1183a4cab5d278e4e0142bc27dea
• Download URL:
  https://stacks.cdc.gov/view/cdc/199057/cdc_199057_DS1.pdf
• File Type:
  [PDF - 595.40 KB ]