Comparison of Surface Sampling Methods for Bacillus anthracis Spore Contamination

Details

• Corporate Authors:
  National Institute for Occupational Safety and Health
• Description:
  Sampling to determine the presence of Bacillus anthracis spores in an environment is important for determining extent of contamination, assessing risk for exposure and need for medical treatment, and especially for guiding clean-up and re-entry efforts. There are currently no validated methods specifically for collecting and analyzing B. anthracis spores in environmental samples. In this investigation, samples were collected in a U.S. Postal facility in Washington, DC which was known to be extensively contaminated with B. anthracis spores. One of the objectives of this survey was to compare the relative effectiveness of surface swab, wipe, and HEPA vacuum samples for collecting spores from contaminated surfaces. The results of this side-by-side comparison of swab (wet and dry), wipe, and HEPA vacuum samples on non-porous surfaces indicated good agreement between the HEPA vacuum and wipe samples. However, the HEPA vacuum sock and wipe samples agreed poorly with the swab samples. The dry swabs performed especially poorly, failing to detect spores over two thirds of the time when they were detected by wipe and HEPA vacuum samples. This data indicate that dry swabs should be not be used as a sampling procedure for B. anthracis spores. The wipe samples collected following HEPA vacuum samples and the HEPA vacuum samples following wipe samples indicated that neither of these sampling methods completely removed spores from the sampled surfaces. This study provides evidence for the need to quantify sampling efficiency to develop the type of "limit of detection data" that are normally created for other types of sampling and analytical methods. [Description provided by NIOSH]
• Subjects:
  Biological Products Biological Warfare Agents Environmental Exposure Environmental Pollution Hazardous Substances Microbiology Occupational Health Safety
• Keywords:
  Biohazards Biological-agents Biological-warfare-agents Biological-weapons Environmental-contamination Environmental-hazards Microorganisms Postal-employees Sampling Sampling-equipment Sampling-methods Surface-properties Vacuum-equipment

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• Document Type:
  Text
• Genre:
  Report
• Place as Subject:
  District of Columbia ; Georgia ; Ohio ; OSHA Region 3 ; OSHA Region 4 ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DSHEFS - Division of Surveillance, Hazard Evaluations, and Field Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  1-25
• NIOSHTIC Number:
  nn:20021995
• Citation:
  NIOSH 2002 Jun; :1-25
• Federal Fiscal Year:
  2002
• Peer Reviewed:
  False
• Source Full Name:
  National Institute for Occupational Safety and Health
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7dc847c09aedf140bc731323e8446b4703641ae07d6ad9e7b7238120dab30b8f7dcaa9b938d34d035f87873a0fe7fec262d5d13dcaf290925445e82b6de3b57d
• Download URL:
  https://stacks.cdc.gov/view/cdc/199021/cdc_199021_DS1.pdf
• File Type:
  [PDF - 168.02 KB ]