Quantitative Analysis of Phospholipid Peroxidation and Antioxidant Protection in Live Human Epidermal Keratinocytes

Details

• Personal Author:
  Kagan VE ; Kikuchi Y ; Quinn PJ ; Shvedova AA ; Tyurin VA ; Tyurina YY
• Description:
  To characterize oxidative stress in phospholipids of normal human epidermal keratinocytes we metabolically labeled their membrane phospholipids with a natural oxidation-sensitive fluorescent fatty acid, cis-parinaric acid, and exposed the cells to two different sources of oxidants--a lipid-soluble azo-initiator of peroxyl radicals, 2,2'-azobis(2,4-dimethyl-valeronitrile), AMVN, and a superoxide generator, xanthine oxidase/xanthine. We demonstrated that both oxidants induced pronounced oxidation of four major classes of cis-parinaric acid-labeled phospholipids-phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and phosphatidylinositol-in normal human epidermal keratinocytes that was not detectable as any significant change of their phospholipid composition. Vitamin E was effective in protecting the cells against phospholipid peroxidation. Since viability of normal human epidermal keratinocytes was not changed either by labeling or exposure to oxidants the labeling protocol and oxidative stress employed are compatible with the quantitative analysis of phospholipid peroxidation in viable cells. [Description provided by NIOSH]
• Subjects:
  Antioxidants Carcinogens Lipids Occupational Health Safety Skin
• Keywords:
  Alpha-tocopherol AMVN Author Keywords: Keratinocytes Carcinogenesis Exposure-levels Oxidative Stress Phospholipid Peroxidation Phospholipids Stress Xanthine Xanthine Oxidase

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• ISSN:
  0144-8463
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; Pennsylvania ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  33-43
• Volume:
  21
• Issue:
  1
• NIOSHTIC Number:
  nn:20021872
• Citation:
  Biosci Rep 2001 Feb; 21(1):33-43
• Contact Point Address:
  Anna A. Shvedova, Health Effects Laboratory Division, Pathology and Physiology Research Branch, NIOSH, 1095 Willowdale Road, Morgantown, WV 26505
• Federal Fiscal Year:
  2001
• Peer Reviewed:
  True
• Source Full Name:
  Bioscience Reports
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:f945ad78c661cf7981bedfc1ee514322f6b2acf68add4e6b85859a62f972a8664760414cf007e81bd2e4f3e922a602eea12d0216d8bed936d21b407f99c9ca9a
• Download URL:
  https://stacks.cdc.gov/view/cdc/198960/cdc_198960_DS1.pdf
• File Type:
  [PDF - 121.23 KB ]