Detection of Three Novel Translocation and Specific Common Chromosomal Break Sites in Malignant Melanoma by Spectral Karyotyping

Details

• Personal Author:
  Ariza ME ; Jefferson AM ; Lowry DT ; Nelson MA ; Reynolds SH ; Sargent LM ; Senft JR
• Description:
  Chromosomal aberrations in malignant melanoma cells have been reported using standard chromosome banding analysis and comparative genomic hybridization. To identify marker chromosomes and translocations that are difficult to characterize by standard banding analysis, 15 early passage malignant melanoma cell lines were examined using spectral karyotyping. All 15 tumor cell lines had lost all or part of 1p and 10q. Losses of material on chromosome arms 4p (12/15), 6q (12/15), 9p (15/15), 12p (13/15), 12q (13/15), 13q (11/15), and 19q (14/15) were the next most frequent events. Gain of chromosome arms 1q (11/15), 6p (13/15), and 20q11 (14/15) was also observed. Interestingly, we identified translocations der(12)t(12;20)(q15;q11), der(19)t(10;19)(q23;q13), and der(12)t(12;19)(q13;q13) in 4/15 tumors. Three recurring translocations involving four of the most frequent break points were detected. The identification of recurring translocations and unique chromosome break points in melanoma will aid in the identification of the genes that are important in the neoplastic process. [Description provided by NIOSH]
• Subjects:
  Chromosomes Genetics Neoplasms Occupational Health Safety
• Keywords:
  Cancer Chromosome-damage Chromosome-disorders Genes Tumors
• ISSN:
  1045-2257
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Arizona ; OSHA Region 3 ; OSHA Region 9 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  18-25
• Volume:
  32
• Issue:
  1
• NIOSHTIC Number:
  nn:20021864
• Citation:
  Genes Chromosomes Cancer 2001 Sep; 32(1):18-25
• Contact Point Address:
  Dr. Linda M. Sargent, 1095 Willowdale Road, MS-3014, Morgantown, WV 26505
• Email:
  lqsl@cdc.gov
• Editor(s):
  Mitelman F; Rowley JD
• Federal Fiscal Year:
  2001
• Peer Reviewed:
  True
• Source Full Name:
  Genes Chromosomes and Cancer
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:bb1a8b8f12685e606feb45e801f02a0429443f96e9fa3aa3705d2a38f19aa0e65063fac399f2d4f0ec0c9b9ac5f175a2d8c149c1fa239fa5a5f0f5dd334e435a
• Download URL:
  https://stacks.cdc.gov/view/cdc/198954/cdc_198954_DS1.pdf
• File Type:
  [PDF - 572.00 KB ]