Promoter Hypermethylation of DLC-1, a Candidate Tumor Suppressor Gene, in Several Common Human Cancers

Details

• Personal Author:
  Durkin ME ; Popescu NC ; Yuan BZ
• Description:
  Aberrant methylation of CpG islands within the promoter regions of tumor suppressor or cancer-related genes is a common mechanism leading to the silencing of gene expression. To determine whether aberrant methylation is a contributing factor to transcriptional inactivation of DLC-1 (deleted in liver cancer-1), a candidate tumor suppressor gene, we examined its methylation status in twelve hepatocellular carcinoma, breast, colon, and prostate tumor cell lines with low or undetectable expression of DLC-1. By Southern blot analysis of DNA digested with the methylation sensitive enzyme HpaII, we found a different degree of promoter hypermethylation in all cell lines with aberrant DLC-1 expression. The hypermethylation status was reversed by the addition of 5-aza-2'-deoxycytidine, a demethylating agent, in one human hepatocellular carcinoma line. These observations suggest that hypermethylation is responsible for abrogating the function of the DLC-1 gene in a subset of liver, breast, colon, and prostate cancers. [Description provided by NIOSH]
• Subjects:
  Chromosomes Enzymes Genetics Neoplasms Occupational Health Safety
• Keywords:
  Cancer Cell Alteration Cell Morphology Chromosome Damage Genes Liver Tumors Proteins Tumor Inhibition Tumors
• ISSN:
  0165-4608
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Maryland ; OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  113-117
• Volume:
  140
• Issue:
  2
• NIOSHTIC Number:
  nn:20023526
• Citation:
  Cancer Genet Cytogenet 2003 Jan; 140(2):113-117
• Contact Point Address:
  Laboratory of Experimental Carcinogenesis, Building 37 Room 3C05, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
• Email:
  popsecun@mail.nih.gov
• Federal Fiscal Year:
  2003
• Peer Reviewed:
  True
• Source Full Name:
  Cancer Genetics and Cytogenetics
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:818e0e17d1714fc91540ac3c8625237f8eba1930d2bae1ccdea54b151d584efa09efab40aeb00ebd9ece0fc81d3f8a41755098b2758eef9de481a0e067bc6875
• Download URL:
  https://stacks.cdc.gov/view/cdc/195899/cdc_195899_DS1.pdf
• File Type:
  [PDF - 248.59 KB ]