Cr(VI) Increases Tyrosine Phosphorylation Through Reactive Oxygen Species-Mediated Reactions

Details

• Personal Author:
  Chen F ; Flynn DC ; Jiang BH ; Leonard, Stephen S. ; Qian Y ; Shi X ; Wang L ; Wang S ; Ye J ; Zhang Z
• Description:
  While Cr (VI)-containing compounds are well established carcinogens, the mechanisms of their action remain to be investigated. In this study we show that Cr (VI) causes increased tyrosine phosphorylation in human lung epithelial A549 cells in a time-dependent manner. N-acetyl-cysteine (NAC), a general antioxidant, inhibited Cr (VI)-induced tyrosine phosphorylation. Catalase, a scavenger of H2O2, sodium formate and aspirin, scavengers of hydroxyl radical (*OH), also inhibited the increased tyrosine phosphorylation induced by Cr (VI). SOD, an inhibitor of superoxide radical (O2*-), caused less inhibition. ESR study shows that incubation of Cr (VI) with the A549 cells generates *OH radical. The generation of radical was decreased by addition of catalase and sodium formate, while SOD did not have any inhibitory effect. Oxygen consumption measurements show that addition of Cr (VI) to A549 cells resulted in enhanced molecular oxygen consumption. These results indicate that Cr (VI) can induce an increase in tyrosine phosphorylation. H2O2 and *OH radicals generated during the process are responsible for the increased tyrosine phosphorylation induced by Cr (VI). [Description provided by NIOSH]
• Subjects:
  Antioxidants Carcinogens Cells, Cultured Lung Occupational Health Respiratory System Safety
• Keywords:
  A549 Cells Antioxidation Author Keywords: Cr (VI) Cell-cultures Lung-cells Sodium-compounds Tyrosine Phosphorylation
• ISSN:
  0300-8177
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  199-204
• Volume:
  222
• Issue:
  1
• NIOSHTIC Number:
  nn:20021845
• Citation:
  Mol Cell Biochem 2001 Jun; 222(1-2):199-204
• Contact Point Address:
  D. C. Flynn, Cancer Center and Department of Microbiology and Immunology, School of Medicine, West Virginia University, Morgantown, WV 26506
• CAS Registry Number:
  L-Tyrosine (CAS RN 55520-40-6)
• Federal Fiscal Year:
  2001
• Peer Reviewed:
  True
• Source Full Name:
  Molecular and Cellular Biochemistry
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:2cf96d541e7f2669aee68608d29071dd1e4b89d4ca9b7055a756cbc07d80e71afb41bd4213c5cea2d7984ac64d73716c645c9aa90ad1ea191f4b760052cc16fa
• Download URL:
  https://stacks.cdc.gov/view/cdc/198941/cdc_198941_DS1.pdf
• File Type:
  [PDF - 136.38 KB ]