Luteolin and Chrysin Differentially Inhibit Cyclooxygenase-2 Expression and Scavenge Reactive Oxygen Species but Similarly Inhibit Prostaglandin-E2 Formation in RAW 264.7 Cells

Details

• Personal Author:
  Harris GK ; Leonard, Stephen S. ; Qian Y ; Sbarra DC ; Shi X
• Description:
  Inflammation and oxidative stress are associated with cancer, atherosclerosis, and other chronic diseases. Dietary flavonoids have been reported to possess antiinflammatory and antioxidant properties, but their mechanisms of action and structure-activity relations have not been fully investigated. We hypothesized that differences in antioxidant activity between the structurally similar flavones, luteolin and chrysin (differing only in B-ring hydroxylation patterns), would differentially affect inflammation-associated Cox-2 expression and PGE2 formation. Pretreatment of RAW 264.7 macrophage-like cells with 25, 50, or 100 micromol/L concentrations of luteolin inhibited lipopolysaccharide (LPS)-induced Cox-2 protein expression (P < 0.0001). Chrysin pretreatment did not reduce LPS-induced Cox-2 protein expression at any level tested. Conversely, both luteolin and chrysin completely suppressed LPS-induced PGE2 formation (P < 0.001). Luteolin, but not chrysin, inhibited xanthine/xanthine oxidase-generated superoxide formation at 100 micromol/L in a cell-free system (P < 0.001). Although both luteolin and chrysin reduced LPS-induced hydroxyl radical formation relative to the positive control (P < 0.001), luteolin was superior to chrysin (P = 0.003). In summary, luteolin and chrysin suppressed PGE2 formation equally well, despite differential effects on Cox-2 protein expression and on superoxide and hydroxyl radical scavenging. These data indicate that flavones may display similar antiinflammatory activity via different mechanisms. [Description provided by NIOSH]
• Subjects:
  Antioxidants Cells, Cultured Diet Occupational Health Safety
• Keywords:
  Antioxidation Cell-cultures Dietary-effects Flavones Nutrition Nutritional-disorders Proteins
• ISSN:
  0022-3166
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Maryland ; OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  136
• Issue:
  6
• NIOSHTIC Number:
  nn:20030325
• Citation:
  J Nutr 2006 Jun; 136(6):1517-1521
• Email:
  harrisk@ba.ars.usda.gov
• Federal Fiscal Year:
  2006
• NORA Priority Area:
  Research Tools and Approaches: Cancer Research Methods
• Peer Reviewed:
  True
• Source Full Name:
  The Journal of Nutrition
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:86e0557401dafb08e75444c52d7c252ad0d70edda106bb47b0b475182d0d9aca52c7791c45fe0215d964e25baeecbb8105e8338085e5a0eaf17780d5f04b11d9
• Download URL:
  https://stacks.cdc.gov/view/cdc/191008/cdc_191008_DS1.pdf
• File Type:
  [PDF - 236.79 KB ]