Arsenic-Induced NFkB Transactivation Through Erks- and JNKs-Dependent Pathways in Mouse Epidermal JB6 Cells

Details

• Personal Author:
  Castranova, Vincent ; Costa M ; Ding M ; Huang C ; Ju G ; Li J ; Shi X ; Vallyathan V ; Wang L
• Description:
  Tumor promoting effects of arsenic are believed to be associated with its transactivation activity on transcription factors, such as AP-1 and NFkappaB. However, the results from different groups studying the effects of arsenic on NFkappaB activation are contradictory in different cell models. Since arsenic is a strong skin carcinogen, we have investigated the activation of NFkappaB by arsenic in a mouse skin epidermal cell line, JB6 cells. Exposure of cells to arsenite or arsenate led to NFkappaB transactivation in mouse epidermal JB6 NFkappaB-luciferase reporter stable transfectants, C141 NFkappaB mass1. This induction of NFkappaB activity by arsenic was dose- and time-dependent. The transactivation of NFkappaB by arsenic appeared to be through activation of Erks and JNKs pathways because increased NFkappaB activity by arsenic could be dramatically inhibited by either pre-treatment of cells with PD98059 or overexpression of dominant negative JNK1. That Erks activation is required for arsenic-induced NFkappaB transactivation was further supported by the findings that arsenic-induced NFkappaB transactivation was impaired in JB6 30.7b cells, which were deficient in Erks. [Description provided by NIOSH]
• Subjects:
  Animals Arsenic Carcinogens Cells, Cultured Environmental Monitoring Inorganic Chemicals Laboratories Neoplasms Occupational Health Safety Skin Diseases

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• Keywords:
  Animal-studies Arsenic-compounds Author Keywords: Arsenic Cell-cultures Exposure-assessment Exposure-levels Laboratory-animals MAP Kinase Models NFkB Skin-disorders Tumors

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• ISSN:
  0300-8177
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  New York ; OSHA Region 2 ; OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  29-34
• Volume:
  222
• Issue:
  1
• NIOSHTIC Number:
  nn:20021755
• Citation:
  Mol Cell Biochem 2001 Jun; 222(1-2):29-34
• Contact Point Address:
  Nelson Institute of Environmental Medicine, New York University School of Medicine, NY 10016
• CAS Registry Number:
  Arsenic (CAS RN 7440-38-2) ; Arsenite (CAS RN 15502-74-6)
• Federal Fiscal Year:
  2001
• Peer Reviewed:
  True
• Source Full Name:
  Molecular and Cellular Biochemistry
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:16a8b8dff50876aa619d552fcd63bda2da86968023c1b975872b4395d11ba25162bdc6d7e6fdc12e674e75a5e6d54bbfcf713a86087531c2ec95f87826b6715b
• Download URL:
  https://stacks.cdc.gov/view/cdc/198864/cdc_198864_DS1.pdf
• File Type:
  [PDF - 109.24 KB ]