Clinical Biological Monitoring Using Immunobiochemical Methods

Details

• Personal Author:
  Biagini, Raymond E. ; Hines CJ ; MacKenzie, Barbara A. ; Snawder, John E. ; Striley CAF
• Description:
  Monitoring of urinary metabolites from pesticide applicators' has been routinely used in NIOSH field studies. Commercially available enzyme linked immunosorbant assays (ELISAs) have been successfully used for screening. Currently we are investigating immunochemical biological monitoring methods for organophosphate pesticides including chlorpyrifos. A magneto-particle ELISA for trichloropyridinol (TCP), a human metabolite of chlorpyrifos, was modified for urinary biomonitoring by beta-glucuronidase pretreatment to yield parent TCP. Biological monitoring for exposure to metolachlor necessitated the development of an ELISA for its putative mercapturic acid metabolite. Development of these biological monitoring strategies entails knowledge of agent specific human pharmacokinetics. We have procured a library of human tissue sub-fractions to serve as an in vitro surrogate of human pharmacokinetics. Each tissue sample has been immunochemically and phentotypically characterized for its toxicokinetic potential. Aspects of inter-individual variation in xenobiotic metabolism can then be evaluated as a covariate in a fully integrated system to develop biomonitoring strategies. [Description provided by NIOSH]
• Subjects:
  Biomarkers Chemistry, Analytic Immune System Insecticides Occupational Health Safety
• Keywords:
  Analytical Chemistry Analytical Processes Biological Monitoring Immunochemistry Organo Phosphorus Pesticides Pesticides Pesticides And Agricultural Chemicals Pharmacodynamics
• ISSN:
  0065-7727
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings
• Place as Subject:
  California ; Ohio ; OSHA Region 5 ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DBBS - Division of Biomedical and Behavioral Sciences ; DSHEFS - Division of Surveillance, Hazard Evaluations, and Field Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  219
• NIOSHTIC Number:
  nn:20021075
• Citation:
  Abstr Pap - Am Chem Soc 2000 Mar; 219:AGRO-U79
• Contact Point Address:
  Cynthia A. F. Striley, Division of Biomedical and Behavioral Science, National Institute for Occupational Safety and Health, 4676 Columbia Parkway, Mailstop C-26, Cincinnati, OH 45226
• Email:
  chs3@cdc.gov
• CAS Registry Number:
  Chlorpyrifos (CAS RN 2921-88-2)
• Federal Fiscal Year:
  2000
• Peer Reviewed:
  False
• Source Full Name:
  Abstracts of Papers - American Chemical Society
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:a277cfe09be1677c2631606643432ae0e4944f5aff6dc887070fd328f06c52b67c0ed60cec9d126d8554084bbec8f6c0963e0969f1829ba86132f737ffe08c7d
• Download URL:
  https://stacks.cdc.gov/view/cdc/198637/cdc_198637_DS1.pdf
• File Type:
  [PDF - 111.80 KB ]