Differences Between Alveolar Macrophages and Cell Lines in Activation of Mitogen-Activated Protein Kinases (MAPKs) Stimulated by Lipo-Polyscccharide (LPS)

Details

• Personal Author:
  Meighan T ; Rao KMK
• Description:
  We have shown previously that the lung surfactant inhibits LPS-stimulated nitric oxide (NO) production in alveolar macrophages (Miles et al., Am.J.Physiol. 276:Ll86, 1999). But lung surfactant had no effect on NO production in two macrophage cell lines RAW 264.7 and J-774. Stimulation of primary macrophages and cell lines by LPS has been shown to activate MAPKs. To determine if differences in MAPK activation may explain the difference in response to lung surfactant between primary macrophages and the cell lines, we compared the activation of MAPKs, p38, extracelluar-regulated kinase (ERK), and c-Jun N-terminal kinase (JNK/SAPK), in rat alveolar macrophages and three macrophage cell lines, RAW 264.7, J-774, and NR8383. In alveolar macrophages there was basal phosphorylation of p38 and ERK which did not significantly increase on LPS stimulation. There was no detectable JNK phosphorylation in unstimulated or LPS-stimulated alveolar macrophages. In contrast, in all three cell lines LPS induced a significant increase in phosphorylation of p38. Differences in activation of MAP kinases may determine the response to lung surfactant. [Description provided by NIOSH]
• Subjects:
  Lung Lung Diseases Occupational Health Respiratory System Respiratory Tract Diseases Safety Surface-Active Agents
• Keywords:
  Alveolar Cells Lung Disorders Proteins Pulmonary System Disorders Respiratory System Disorders Surfactants
• ISSN:
  0892-6638
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  14
• Issue:
  4
• NIOSHTIC Number:
  nn:20021012
• Citation:
  FASEB J 2000 Mar; 14(4):A587
• CAS Registry Number:
  Nitric oxide (CAS RN 10102-43-9)
• Federal Fiscal Year:
  2000
• Peer Reviewed:
  False
• Source Full Name:
  The FASEB Journal
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:34cf341199ef82dd4cec935d8cf21b69df3534502b4f82ce567dd4c8054881251e270c734000c4851d8e90c44df31909090f0713bcbc74bd13a733707d84065e
• Download URL:
  https://stacks.cdc.gov/view/cdc/198603/cdc_198603_DS1.pdf
• File Type:
  [PDF - 170.24 KB ]