Pulmonary Surfactant Inhibits LPS-Induced Nitric Oxide Production by Alveolar Macrophages

Details

• Personal Author:
  Baatz JE ; Bowman L ; Huffman L ; Miles PR ; Rao KMK
• Description:
  The objectives of this investigation were 1) to report that pulmonary surfactant inhibits lipopolysaccharide (LPS)-induced nitric oxide (· NO) production by rat alveolar macrophages, 2) to study possible mechanisms for this effect, and 3) to determine which surfactant component(s) is responsible. · NO produced by the cells in response to LPS is due to an inducible · NO synthase (iNOS). Surfactant inhibits LPS-induced · NO formation in a concentration-dependent manner; · NO production is inhibited by about 50 and about 75% at surfactant levels of 100 and 200 microg phospholipid/ml, respectively. The inhibition is not due to surfactant interference with the interaction of LPS with the cells or to disruption of the formation of iNOS mRNA. Also, surfactant does not seem to reduce · NO formation by directly affecting iNOS activity or by acting as an antioxidant or radical scavenger. However, in the presence of surfactant, there is an about 80% reduction in the amount of LPS-induced iNOS protein in the cells. LPS-induced · NO production is inhibited by Survanta, a surfactant preparation used in replacement therapy, as well as by natural surfactant. · NO formation is not affected by the major lipid components of surfactant or by two surfactant-associated proteins, surfactant protein (SP) A or SP-C. However, the hydrophobic SP-B inhibits · NO formation in a concentration-dependent manner; · NO production is inhibited by about 50 and about 90% at SP-B levels of 1-2 and 10 microg/ml, respectively. These results show that lung surfactant inhibits LPS-induced · NO production by alveolar macrophages, that the effect is due to a reduction in iNOS protein levels, and that the surfactant component responsible for the reduction is SP-B. [Description provided by NIOSH]
• Subjects:
  Hematologic Diseases Lung Lung Diseases Nitrogen Compounds Nitrogen Oxides Nitrous Oxide Occupational Health Respiratory System Respiratory Tract Diseases Safety Surface-Active Agents

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• Keywords:
  Alveolar-cells Author Keywords: Lipopolysaccharide Blood-disorders Hydrophobic Surfactant Proteins Inducible Nitric Oxide Synthase Lung-cells Lung-disorders Lung-fibrosis Nitrogen-compounds Nitrogen-oxides Nitrous-oxides Physiological-chemistry Pulmonary-system-disorders Respiratory-system-disorders Statistical-analysis Surfactant Protein B Surfactants

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• ISSN:
  1040-0605
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; OSHA Region 4 ; South Carolina ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  186-196
• Volume:
  276
• Issue:
  1
• NIOSHTIC Number:
  nn:20027773
• Citation:
  Am J Physiol Lung Cell Mol Physiol 1999 Jan; 276(1):186-196
• Contact Point Address:
  P. R. Miles, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown 26505
• CAS Registry Number:
  Nitric oxide (CAS RN 10102-43-9)
• Federal Fiscal Year:
  1999
• Peer Reviewed:
  True
• Source Full Name:
  American Journal of Physiology: Lung Cellular and Molecular Physiology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:e1ca549a3f166ad9b968021013e0a582d52b5ca5b67fea8c987a8f706625eadef70a6de9084f84df4c49b8a5b04e2aec37609a586a95aba85633b095955eab80
• Download URL:
  https://stacks.cdc.gov/view/cdc/197656/cdc_197656_DS1.pdf
• File Type:
  [PDF - 179.66 KB ]